Patients with type 1 diabetes show signs of vascular dysfunction in response to multiple high-fat meals

Mariann I. Lassenius, Ville Petteri Mäkinen, Christopher L. Fogarty, Lina Peräneva, Matti Jauhiainen, Pirkko J. Pussinen, Marja Riitta Taskinen, Juha Kirveskari, Outi Vaarala, Janne K. Nieminen, Sohvi Hörkkö, Antti J. Kangas, Pasi Soininen, Mika Ala-Korpela, Daniel Gordin, Aila J. Ahola, Carol Forsblom, Per Henrik Groop, Markku Lehto

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: A high-fat diet promotes postprandial systemic inflammation and metabolic endotoxemia. We investigated the effects of three consecutive high-fat meals on endotoxemia, inflammation, vascular function, and postprandial lipid metabolism in patients with type 1 diabetes. Methods. Non-diabetic controls (n = 34) and patients with type 1 diabetes (n = 37) were given three high-caloric, fat-containing meals during one day. Blood samples were drawn at fasting (8:00) and every two hours thereafter until 18:00. Applanation tonometry was used to assess changes in the augmentation index during the investigation day. Results: Three consecutive high-fat meals had only a modest effect on serum LPS-activity levels and inflammatory markers throughout the day in both groups. Of note, patients with type 1 diabetes were unable to decrease the augmentation index in response to the high-fat meals. The most profound effects of the consecutive fat loads were seen in chylomicron and HDL-metabolism. The triglyceride-rich lipoprotein remnant marker, apoB-48, was elevated in patients compared to controls both at fasting (p = 0.014) and postprandially (p = 0.035). The activities of the HDL-associated enzymes PLTP (p < 0.001), and CETP (p = 0.007) were higher and paraoxonase (PON-1) activity, an anti-oxidative enzyme bound to HDL, decreased in patients with type 1 diabetes (p = 0.027). Conclusions: In response to high-fat meals, early signs of vascular dysfunction alongside accumulation of chylomicron remnants, higher augmentation index, and decreased PON-1 activity were observed in patients with type 1 diabetes. The high-fat meals had no significant impact on postprandial LPS-activity in non-diabetic subjects or patients with type 1 diabetes suggesting that metabolic endotoxemia may be more central in patients with chronic metabolic disturbances such as obesity, type 2 diabetes, or diabetic kidney disease.

LanguageEnglish
Article number28
JournalNutrition and Metabolism
Volume11
Issue number1
DOIs
Publication statusPublished - 13 Jun 2014

Keywords

  • High-fat diet
  • Type 1 diabetes
  • Vascular dysfunction

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Lassenius, Mariann I. ; Mäkinen, Ville Petteri ; Fogarty, Christopher L. ; Peräneva, Lina ; Jauhiainen, Matti ; Pussinen, Pirkko J. ; Taskinen, Marja Riitta ; Kirveskari, Juha ; Vaarala, Outi ; Nieminen, Janne K. ; Hörkkö, Sohvi ; Kangas, Antti J. ; Soininen, Pasi ; Ala-Korpela, Mika ; Gordin, Daniel ; Ahola, Aila J. ; Forsblom, Carol ; Groop, Per Henrik ; Lehto, Markku. / Patients with type 1 diabetes show signs of vascular dysfunction in response to multiple high-fat meals. In: Nutrition and Metabolism. 2014 ; Vol. 11, No. 1.
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abstract = "Background: A high-fat diet promotes postprandial systemic inflammation and metabolic endotoxemia. We investigated the effects of three consecutive high-fat meals on endotoxemia, inflammation, vascular function, and postprandial lipid metabolism in patients with type 1 diabetes. Methods. Non-diabetic controls (n = 34) and patients with type 1 diabetes (n = 37) were given three high-caloric, fat-containing meals during one day. Blood samples were drawn at fasting (8:00) and every two hours thereafter until 18:00. Applanation tonometry was used to assess changes in the augmentation index during the investigation day. Results: Three consecutive high-fat meals had only a modest effect on serum LPS-activity levels and inflammatory markers throughout the day in both groups. Of note, patients with type 1 diabetes were unable to decrease the augmentation index in response to the high-fat meals. The most profound effects of the consecutive fat loads were seen in chylomicron and HDL-metabolism. The triglyceride-rich lipoprotein remnant marker, apoB-48, was elevated in patients compared to controls both at fasting (p = 0.014) and postprandially (p = 0.035). The activities of the HDL-associated enzymes PLTP (p < 0.001), and CETP (p = 0.007) were higher and paraoxonase (PON-1) activity, an anti-oxidative enzyme bound to HDL, decreased in patients with type 1 diabetes (p = 0.027). Conclusions: In response to high-fat meals, early signs of vascular dysfunction alongside accumulation of chylomicron remnants, higher augmentation index, and decreased PON-1 activity were observed in patients with type 1 diabetes. The high-fat meals had no significant impact on postprandial LPS-activity in non-diabetic subjects or patients with type 1 diabetes suggesting that metabolic endotoxemia may be more central in patients with chronic metabolic disturbances such as obesity, type 2 diabetes, or diabetic kidney disease.",
keywords = "High-fat diet, Type 1 diabetes, Vascular dysfunction",
author = "Lassenius, {Mariann I.} and M{\"a}kinen, {Ville Petteri} and Fogarty, {Christopher L.} and Lina Per{\"a}neva and Matti Jauhiainen and Pussinen, {Pirkko J.} and Taskinen, {Marja Riitta} and Juha Kirveskari and Outi Vaarala and Nieminen, {Janne K.} and Sohvi H{\"o}rkk{\"o} and Kangas, {Antti J.} and Pasi Soininen and Mika Ala-Korpela and Daniel Gordin and Ahola, {Aila J.} and Carol Forsblom and Groop, {Per Henrik} and Markku Lehto",
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Lassenius, MI, Mäkinen, VP, Fogarty, CL, Peräneva, L, Jauhiainen, M, Pussinen, PJ, Taskinen, MR, Kirveskari, J, Vaarala, O, Nieminen, JK, Hörkkö, S, Kangas, AJ, Soininen, P, Ala-Korpela, M, Gordin, D, Ahola, AJ, Forsblom, C, Groop, PH & Lehto, M 2014, 'Patients with type 1 diabetes show signs of vascular dysfunction in response to multiple high-fat meals', Nutrition and Metabolism, vol. 11, no. 1, 28. https://doi.org/10.1186/1743-7075-11-28

Patients with type 1 diabetes show signs of vascular dysfunction in response to multiple high-fat meals. / Lassenius, Mariann I.; Mäkinen, Ville Petteri; Fogarty, Christopher L.; Peräneva, Lina; Jauhiainen, Matti; Pussinen, Pirkko J.; Taskinen, Marja Riitta; Kirveskari, Juha; Vaarala, Outi; Nieminen, Janne K.; Hörkkö, Sohvi; Kangas, Antti J.; Soininen, Pasi; Ala-Korpela, Mika; Gordin, Daniel; Ahola, Aila J.; Forsblom, Carol; Groop, Per Henrik; Lehto, Markku.

In: Nutrition and Metabolism, Vol. 11, No. 1, 28, 13.06.2014.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Patients with type 1 diabetes show signs of vascular dysfunction in response to multiple high-fat meals

AU - Lassenius, Mariann I.

AU - Mäkinen, Ville Petteri

AU - Fogarty, Christopher L.

AU - Peräneva, Lina

AU - Jauhiainen, Matti

AU - Pussinen, Pirkko J.

AU - Taskinen, Marja Riitta

AU - Kirveskari, Juha

AU - Vaarala, Outi

AU - Nieminen, Janne K.

AU - Hörkkö, Sohvi

AU - Kangas, Antti J.

AU - Soininen, Pasi

AU - Ala-Korpela, Mika

AU - Gordin, Daniel

AU - Ahola, Aila J.

AU - Forsblom, Carol

AU - Groop, Per Henrik

AU - Lehto, Markku

PY - 2014/6/13

Y1 - 2014/6/13

N2 - Background: A high-fat diet promotes postprandial systemic inflammation and metabolic endotoxemia. We investigated the effects of three consecutive high-fat meals on endotoxemia, inflammation, vascular function, and postprandial lipid metabolism in patients with type 1 diabetes. Methods. Non-diabetic controls (n = 34) and patients with type 1 diabetes (n = 37) were given three high-caloric, fat-containing meals during one day. Blood samples were drawn at fasting (8:00) and every two hours thereafter until 18:00. Applanation tonometry was used to assess changes in the augmentation index during the investigation day. Results: Three consecutive high-fat meals had only a modest effect on serum LPS-activity levels and inflammatory markers throughout the day in both groups. Of note, patients with type 1 diabetes were unable to decrease the augmentation index in response to the high-fat meals. The most profound effects of the consecutive fat loads were seen in chylomicron and HDL-metabolism. The triglyceride-rich lipoprotein remnant marker, apoB-48, was elevated in patients compared to controls both at fasting (p = 0.014) and postprandially (p = 0.035). The activities of the HDL-associated enzymes PLTP (p < 0.001), and CETP (p = 0.007) were higher and paraoxonase (PON-1) activity, an anti-oxidative enzyme bound to HDL, decreased in patients with type 1 diabetes (p = 0.027). Conclusions: In response to high-fat meals, early signs of vascular dysfunction alongside accumulation of chylomicron remnants, higher augmentation index, and decreased PON-1 activity were observed in patients with type 1 diabetes. The high-fat meals had no significant impact on postprandial LPS-activity in non-diabetic subjects or patients with type 1 diabetes suggesting that metabolic endotoxemia may be more central in patients with chronic metabolic disturbances such as obesity, type 2 diabetes, or diabetic kidney disease.

AB - Background: A high-fat diet promotes postprandial systemic inflammation and metabolic endotoxemia. We investigated the effects of three consecutive high-fat meals on endotoxemia, inflammation, vascular function, and postprandial lipid metabolism in patients with type 1 diabetes. Methods. Non-diabetic controls (n = 34) and patients with type 1 diabetes (n = 37) were given three high-caloric, fat-containing meals during one day. Blood samples were drawn at fasting (8:00) and every two hours thereafter until 18:00. Applanation tonometry was used to assess changes in the augmentation index during the investigation day. Results: Three consecutive high-fat meals had only a modest effect on serum LPS-activity levels and inflammatory markers throughout the day in both groups. Of note, patients with type 1 diabetes were unable to decrease the augmentation index in response to the high-fat meals. The most profound effects of the consecutive fat loads were seen in chylomicron and HDL-metabolism. The triglyceride-rich lipoprotein remnant marker, apoB-48, was elevated in patients compared to controls both at fasting (p = 0.014) and postprandially (p = 0.035). The activities of the HDL-associated enzymes PLTP (p < 0.001), and CETP (p = 0.007) were higher and paraoxonase (PON-1) activity, an anti-oxidative enzyme bound to HDL, decreased in patients with type 1 diabetes (p = 0.027). Conclusions: In response to high-fat meals, early signs of vascular dysfunction alongside accumulation of chylomicron remnants, higher augmentation index, and decreased PON-1 activity were observed in patients with type 1 diabetes. The high-fat meals had no significant impact on postprandial LPS-activity in non-diabetic subjects or patients with type 1 diabetes suggesting that metabolic endotoxemia may be more central in patients with chronic metabolic disturbances such as obesity, type 2 diabetes, or diabetic kidney disease.

KW - High-fat diet

KW - Type 1 diabetes

KW - Vascular dysfunction

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U2 - 10.1186/1743-7075-11-28

DO - 10.1186/1743-7075-11-28

M3 - Article

VL - 11

JO - Nutrition and Metabolism

T2 - Nutrition and Metabolism

JF - Nutrition and Metabolism

SN - 1743-7075

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