Oxygen sensing mechanisms are present in the chromaffin cells of the sheep adrenal medulla before birth

G. Y. Rychkov, M. B. Adams, I. C. McMillen, M. L. Roberts

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Abstract

1. The ability of the fetal adrenal medulla to respond directly to hypoxaemia and secrete catecholamines before the development of a functional innervation of the gland is essential for intrauterine survival. The cellular mechanisms involved in this response to low P(O2) are not known, although the presence of oxygen-sensitive K+ channels in carotid body chemoreceptor cells and other sites suggests that these might underlie the chromaffin cell response. 2. Whole-cell patch-clamp techniques have been used to study K+ currents during normoxia and hypoxia in chromaffin cells isolated from the adrenal glands of fetal sheep. 3. Two types of chromaffin cells were observed, those with a fast inactivating K+ current and a larger capacitance and those with a delayed K+ current and smaller capacitance. No cell showed both types of current. The fast inactivating current showed voltage-dependent inactivation and was blocked by 1 mM 4-aminopyridine, characteristics of an I(A)-type current. The delayed current had two components, a TEA-sensitive, Ca2+-dependent current and a component with tile kinetic behaviour of a delayed rectifier. 4. Both types of current were oxygen sensitive. The I(A)-type current was reduced by 27.4 ± 3.2% when the P(O2) was reduced to about 15 mmHg. With the delayed current, hypoxia reduced the amplitude by 26.9 ± 2.4%, largely by reduction of the Ca2+-dependent component. 5. In the presence of hypoxia, reduction in the amplitude of these oxygen-sensitive K+ currents would increase the frequency and duration of action potentials, leading to increased activation of the L-type Ca2+ channels, influx of Ca2+ and the subsequent secretion of catecholamines.

Original languageEnglish
Pages (from-to)887-893
Number of pages7
JournalJournal of Physiology
Volume509
Issue number3
DOIs
Publication statusPublished - 15 Jun 1998

ASJC Scopus subject areas

  • Physiology

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