Oxidation of low-density lipoproteins: Intraindividual variability and the effect of dietary linoleate supplementation

M. Abbey, G. B. Belling, M. Noakes, F. Hirata, P. J. Nestel

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Low-density lipoprotein (LDL) oxidation was measured in vitro to determine intraindividual variability and to relate oxidation to linoleic acid enrichment. Intraindividual variability was determined for eight subjects on 3 consecutive days after 14 d on a fixed diet. Coefficients of variation were 7.49 ± 1.50%, 6.58 ± 1.16%, and 4.58 ± 0.77% for oxidation rate, lag time, and diene concentration, respectively. In the second study 12 normolipidemic men consumed a daily diet supplement containing 35 g linoleate-rich oil in one period and 35 g oleate-rich oil in the other period (2 x 3 wk crossover). LDL oxidized faster after the linoleate diet than after the oleate diet (x̄ ± SE: 16.42 ± 0.85 and 13.16 ± 0.68 nmol diene · mg LDL protein-1 · min-1, respectively, P < 0.02) and produced more conjugated diene (416 ± 12.60 and 379.29 ± 11.06 nmol/mg protein, respectively, P < 0.05) in proportion to the increase in LDL linoleate (r = 0.698, P < 0.001 and r = 0.618, P < 0.01 for rate and diene concentration, respectively). Lag time before onset of oxidation was not significantly altered by the linoleate- rich diet.

Number of pages8
JournalAmerican Journal of Clinical Nutrition
Issue number3
Publication statusPublished - 1 Jan 1993


  • Oxidized low-density lipoprotein
  • antioxidants
  • conjugated dienes
  • copper oxidation
  • monounsaturated fatty acids
  • plasma lipids
  • polyunsaturated fatty acids

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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