Opposing effects of omega-3 and omega-6 long chain polyunsaturated fatty acids on the expression of lipogenic genes in omental and retroperitoneal adipose depots in the rat

B. S. Muhlhausler, R. Cook-Johnson, M. James, D. Miljkovic, E. Duthoit, R. Gibson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, α -linolenic acid (ALA; 18: 3n-3), on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095%en to 6.3%en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid- Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor- γ (PPAR γ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations (omental, r 2 =.71; P<.001; Retroperitoneal, r 2 =.20; P<.002), and inversely to n-3 PUFA concentrations (omental, r 2 =.59; P<.001; Retroperitoneal, r 2 =.19; P<.005) independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.

LanguageEnglish
Article number927836
JournalJournal of Nutrition and Metabolism
Volume2010
DOIs
Publication statusPublished - 2010
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Food Science
  • Nutrition and Dietetics

Cite this

@article{89416b1c1d0a497ab89b77c8ab4cbb1c,
title = "Opposing effects of omega-3 and omega-6 long chain polyunsaturated fatty acids on the expression of lipogenic genes in omental and retroperitoneal adipose depots in the rat",
abstract = "This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, α -linolenic acid (ALA; 18: 3n-3), on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095{\%}en to 6.3{\%}en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid- Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor- γ (PPAR γ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations (omental, r 2 =.71; P<.001; Retroperitoneal, r 2 =.20; P<.002), and inversely to n-3 PUFA concentrations (omental, r 2 =.59; P<.001; Retroperitoneal, r 2 =.19; P<.005) independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.",
author = "Muhlhausler, {B. S.} and R. Cook-Johnson and M. James and D. Miljkovic and E. Duthoit and R. Gibson",
year = "2010",
doi = "10.1155/2010/927836",
language = "English",
volume = "2010",
journal = "Journal of Nutrition and Metabolism",
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T1 - Opposing effects of omega-3 and omega-6 long chain polyunsaturated fatty acids on the expression of lipogenic genes in omental and retroperitoneal adipose depots in the rat

AU - Muhlhausler, B. S.

AU - Cook-Johnson, R.

AU - James, M.

AU - Miljkovic, D.

AU - Duthoit, E.

AU - Gibson, R.

PY - 2010

Y1 - 2010

N2 - This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, α -linolenic acid (ALA; 18: 3n-3), on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095%en to 6.3%en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid- Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor- γ (PPAR γ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations (omental, r 2 =.71; P<.001; Retroperitoneal, r 2 =.20; P<.002), and inversely to n-3 PUFA concentrations (omental, r 2 =.59; P<.001; Retroperitoneal, r 2 =.19; P<.005) independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.

AB - This study aimed to determine the effect of varying dietary intake of the major n-3 PUFA in human diets, α -linolenic acid (ALA; 18: 3n-3), on expression of lipogenic genes in adipose tissue. Rats were fed diets containing from 0.095%en to 6.3%en ALA and a constant n-6 PUFA level for 3 weeks. Samples from distinct adipose depots (omental and retroperitoneal) were collected and mRNA expression of the pro-lipogenic transcription factors Sterol-Retinoid- Element-Binding-Protein1c (SREBP1c) and Peroxisome Proliferator Activated Receptor- γ (PPAR γ), lipogenic enzymes Sterol-coenzyme Desaturase1 (SCD-1), Fatty Acid Synthase (FAS), lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (G3PDH) and adipokines leptin and adiponectin determined by qRT-PCR. Increasing dietary ALA content resulted in altered expression of SREBP1c, FAS and G3PDH mRNA in both adipose depots. SREBP1c mRNA expression was related directly to n-6 PUFA concentrations (omental, r 2 =.71; P<.001; Retroperitoneal, r 2 =.20; P<.002), and inversely to n-3 PUFA concentrations (omental, r 2 =.59; P<.001; Retroperitoneal, r 2 =.19; P<.005) independent of diet. The relationship between total n-6 PUFA and SREBP1c mRNA expression persisted when the effects of n-3 PUFA were controlled for. Altering red blood cell concentrations of n-3 PUFA is thus associated with altered expression of lipogenic genes in a depot-specific manner and this effect is modulated by prevailing n-6 PUFA concentrations.

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U2 - 10.1155/2010/927836

DO - 10.1155/2010/927836

M3 - Article

VL - 2010

JO - Journal of Nutrition and Metabolism

T2 - Journal of Nutrition and Metabolism

JF - Journal of Nutrition and Metabolism

SN - 2090-0724

M1 - 927836

ER -