Novel molecular tumour classification using MALDI-mass spectrometry imaging of tissue micro-array

Marie Claude Djidja, Emmanuelle Claude, Marten F. Snel, Simona Francese, Peter Scriven, Vikki Carolan, Malcolm R. Clench

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

The development of tissue micro-array (TMA) technologies provides insights into high-throughput analysis of proteomics patterns from a large number of archived tumour samples. In the work reported here, matrix-assisted laser desorption/ionisation-ion mobility separation-mass spectrometry (MALDI-IMS-MS) profiling and imaging methodology has been used to visualise the distribution of several peptides and identify them directly from TMA sections after on-tissue tryptic digestion. A novel approach that combines MALDI-IMS-MSI and principal component analysis-discriminant analysis (PCA-DA) is described, which has the aim of generating tumour classification models based on protein profile patterns. The molecular classification models obtained by PCA-DA have been validated by applying the same statistical analysis to other tissue cores and patient samples. The ability to correlate proteomic information obtained from samples with known and/or unknown clinical outcome by statistical analysis is of great importance, since it may lead to a better understanding of tumour progression and aggressiveness and hence improve diagnosis, prognosis as well as therapeutic treatments. The selectivity, robustness and current limitations of the methodology are discussed.

LanguageEnglish
Pages587-601
Number of pages15
JournalAnalytical and Bioanalytical Chemistry
Volume397
Issue number2
DOIs
Publication statusPublished - 1 May 2010
Externally publishedYes

Keywords

  • Ion mobility separation
  • MALDI imaging
  • Pancreatic cancer
  • Tissue micro-array
  • Tumour classification

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Djidja, Marie Claude ; Claude, Emmanuelle ; Snel, Marten F. ; Francese, Simona ; Scriven, Peter ; Carolan, Vikki ; Clench, Malcolm R. / Novel molecular tumour classification using MALDI-mass spectrometry imaging of tissue micro-array. In: Analytical and Bioanalytical Chemistry. 2010 ; Vol. 397, No. 2. pp. 587-601.
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Novel molecular tumour classification using MALDI-mass spectrometry imaging of tissue micro-array. / Djidja, Marie Claude; Claude, Emmanuelle; Snel, Marten F.; Francese, Simona; Scriven, Peter; Carolan, Vikki; Clench, Malcolm R.

In: Analytical and Bioanalytical Chemistry, Vol. 397, No. 2, 01.05.2010, p. 587-601.

Research output: Contribution to journalArticle

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