Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: Impact of age and sex

Hui Li, Claudine L. Frisby, Tracey A. O'Donnell, Stephen J. Kentish, Gary A. Wittert, Amanda J. Page

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aim Gastric vagal afferents are activated in response to mechanical stimulation, an effect attenuated by neuropeptide W (NPW) in 20-week-old female mice. In this study we aimed to determine whether there were age and sex dependent effects of NPW on gastric vagal afferent mechanosensitivity. Methods An in vitro gastro-oesophageal preparation was used to determine the effect of NPW on gastric vagal afferent mechanosensitivity from 8 and 20-week-old male and female C57BL/6 mice. Retrograde tracing and laser capture microdissection were used to selectively collect gastric vagal afferent cell bodies. Expression of NPW in the gastric mucosa and its receptor, GPR7, in gastric vagal afferent cell bodies was determined using quantitative RT-PCR. Results NPW inhibited gastric tension sensitive vagal afferents from 20-week-old male and female mice, but not 8-week-old mice. In contrast, NPW inhibited the mechanosensitivity of gastric mucosal vagal afferents in 8-week-old male and female mice, but not 20-week-old mice. NPW mRNA expression in the gastric mucosa was higher in 20-week-old male mice compared to 8-week-old male mice. GPR7 mRNA expression in vagal afferent neurons innervating the gastric muscular layers was higher in 20-week-old mice compared to 8-week-old mice in both sexes. Conclusion The inhibitory effect of NPW on gastric tension sensitive and mucosal vagal afferents is age but not sex-dependent. These findings suggest that the physiological role of NPW varies depending on the age of the mice.

LanguageEnglish
Pages141-148
Number of pages8
JournalPeptides
Volume71
DOIs
Publication statusPublished - 1 Aug 2015

Keywords

  • Age
  • Neuropeptide W
  • Sex
  • Vagal afferents

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

@article{c4f68aed2ed94ffaa511de196b75c670,
title = "Neuropeptide W modulation of gastric vagal afferent mechanosensitivity: Impact of age and sex",
abstract = "Aim Gastric vagal afferents are activated in response to mechanical stimulation, an effect attenuated by neuropeptide W (NPW) in 20-week-old female mice. In this study we aimed to determine whether there were age and sex dependent effects of NPW on gastric vagal afferent mechanosensitivity. Methods An in vitro gastro-oesophageal preparation was used to determine the effect of NPW on gastric vagal afferent mechanosensitivity from 8 and 20-week-old male and female C57BL/6 mice. Retrograde tracing and laser capture microdissection were used to selectively collect gastric vagal afferent cell bodies. Expression of NPW in the gastric mucosa and its receptor, GPR7, in gastric vagal afferent cell bodies was determined using quantitative RT-PCR. Results NPW inhibited gastric tension sensitive vagal afferents from 20-week-old male and female mice, but not 8-week-old mice. In contrast, NPW inhibited the mechanosensitivity of gastric mucosal vagal afferents in 8-week-old male and female mice, but not 20-week-old mice. NPW mRNA expression in the gastric mucosa was higher in 20-week-old male mice compared to 8-week-old male mice. GPR7 mRNA expression in vagal afferent neurons innervating the gastric muscular layers was higher in 20-week-old mice compared to 8-week-old mice in both sexes. Conclusion The inhibitory effect of NPW on gastric tension sensitive and mucosal vagal afferents is age but not sex-dependent. These findings suggest that the physiological role of NPW varies depending on the age of the mice.",
keywords = "Age, Neuropeptide W, Sex, Vagal afferents",
author = "Hui Li and Frisby, {Claudine L.} and O'Donnell, {Tracey A.} and Kentish, {Stephen J.} and Wittert, {Gary A.} and Page, {Amanda J.}",
year = "2015",
month = "8",
day = "1",
doi = "10.1016/j.peptides.2015.07.011",
language = "English",
volume = "71",
pages = "141--148",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",

}

Neuropeptide W modulation of gastric vagal afferent mechanosensitivity : Impact of age and sex. / Li, Hui; Frisby, Claudine L.; O'Donnell, Tracey A.; Kentish, Stephen J.; Wittert, Gary A.; Page, Amanda J.

In: Peptides, Vol. 71, 01.08.2015, p. 141-148.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Neuropeptide W modulation of gastric vagal afferent mechanosensitivity

T2 - Peptides

AU - Li, Hui

AU - Frisby, Claudine L.

AU - O'Donnell, Tracey A.

AU - Kentish, Stephen J.

AU - Wittert, Gary A.

AU - Page, Amanda J.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Aim Gastric vagal afferents are activated in response to mechanical stimulation, an effect attenuated by neuropeptide W (NPW) in 20-week-old female mice. In this study we aimed to determine whether there were age and sex dependent effects of NPW on gastric vagal afferent mechanosensitivity. Methods An in vitro gastro-oesophageal preparation was used to determine the effect of NPW on gastric vagal afferent mechanosensitivity from 8 and 20-week-old male and female C57BL/6 mice. Retrograde tracing and laser capture microdissection were used to selectively collect gastric vagal afferent cell bodies. Expression of NPW in the gastric mucosa and its receptor, GPR7, in gastric vagal afferent cell bodies was determined using quantitative RT-PCR. Results NPW inhibited gastric tension sensitive vagal afferents from 20-week-old male and female mice, but not 8-week-old mice. In contrast, NPW inhibited the mechanosensitivity of gastric mucosal vagal afferents in 8-week-old male and female mice, but not 20-week-old mice. NPW mRNA expression in the gastric mucosa was higher in 20-week-old male mice compared to 8-week-old male mice. GPR7 mRNA expression in vagal afferent neurons innervating the gastric muscular layers was higher in 20-week-old mice compared to 8-week-old mice in both sexes. Conclusion The inhibitory effect of NPW on gastric tension sensitive and mucosal vagal afferents is age but not sex-dependent. These findings suggest that the physiological role of NPW varies depending on the age of the mice.

AB - Aim Gastric vagal afferents are activated in response to mechanical stimulation, an effect attenuated by neuropeptide W (NPW) in 20-week-old female mice. In this study we aimed to determine whether there were age and sex dependent effects of NPW on gastric vagal afferent mechanosensitivity. Methods An in vitro gastro-oesophageal preparation was used to determine the effect of NPW on gastric vagal afferent mechanosensitivity from 8 and 20-week-old male and female C57BL/6 mice. Retrograde tracing and laser capture microdissection were used to selectively collect gastric vagal afferent cell bodies. Expression of NPW in the gastric mucosa and its receptor, GPR7, in gastric vagal afferent cell bodies was determined using quantitative RT-PCR. Results NPW inhibited gastric tension sensitive vagal afferents from 20-week-old male and female mice, but not 8-week-old mice. In contrast, NPW inhibited the mechanosensitivity of gastric mucosal vagal afferents in 8-week-old male and female mice, but not 20-week-old mice. NPW mRNA expression in the gastric mucosa was higher in 20-week-old male mice compared to 8-week-old male mice. GPR7 mRNA expression in vagal afferent neurons innervating the gastric muscular layers was higher in 20-week-old mice compared to 8-week-old mice in both sexes. Conclusion The inhibitory effect of NPW on gastric tension sensitive and mucosal vagal afferents is age but not sex-dependent. These findings suggest that the physiological role of NPW varies depending on the age of the mice.

KW - Age

KW - Neuropeptide W

KW - Sex

KW - Vagal afferents

UR - http://www.scopus.com/inward/record.url?scp=84938340373&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2015.07.011

DO - 10.1016/j.peptides.2015.07.011

M3 - Article

VL - 71

SP - 141

EP - 148

JO - Peptides

JF - Peptides

SN - 0196-9781

ER -