Multiple genetic loci for zinc uptake and distribution in barley (Hordeum vulgare)

Paul F. Lonergan, Margaret A. Pallotta, Michelle Lorimer, Jeffrey G. Paull, Susan J. Barker, Robin D. Graham

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Summary: Micronutrient malnutrition, often called 'hidden hunger', affects over two billion people globally. This is particularly problematic in developing countries where widespread zinc (Zn) deficiency exists as a result of a predominantly plant-based diet. Furthermore, supplemental fertilizers are often unavailable or unaffordable in impoverished regions where soil infertility is common. Delivery of more Zn via food grains is theoretically possible through selective breeding strategies, but severe technical difficulties associated with trace element research have limited research on the underlying genetic components of Zn nutrition. • Genetic dissection of Zn nutrition involved a pre-existing doubled haploid mapping population of barley (Hordeum vulgare). Association of mineral nutrient accumulation traits with regions of the barley genome was determined in two seasons of growth to maturity, using mapmanager qtx and QGene 4.0. • Nine genetic loci segregating in the population associated clearly with measured traits, including five that contributed to grain Zn status. Pooling two-row doubled haploids by selecting the three most favourable alleles increased grain Zn content and concentration by an average of 53 and 75%, respectively. • These results will inform breeding efforts for increased Zn density in the major food grain, wheat (Triticum aestivum), by enabling syntenic marker-assisted selection in conventional breeding programmes.

Original languageEnglish
Pages (from-to)168-179
Number of pages12
JournalNew Phytologist
Volume184
Issue number1
DOIs
Publication statusPublished - 1 Oct 2009

Keywords

  • Barley (Hordeum vulgare)
  • Doubled haploid population
  • Inductively coupled plasma atomic emission spectrometry (ICPAES)
  • Micronutrient genetics
  • Molecular markers
  • Quantitative trait loci (QTLs)
  • Zinc accumulation
  • Zinc deficiency

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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