Abstract
Monoclonal antibodies have proved to be highly specific tools for defining the antigenic epitopes of Pseudomonas aeruginosa outer membrane macromolecules. In this article we have highlighted the use of monoclonal antibodies in the study of lipopolysaccharide heterogeneity and in particular have demonstrated that single monoclonal antibodies can recognize epitopes on lipid A which are conserved in all Gram negative bacteria tested. Monoclonal antibodies against P. aeruginosa outer membrane proteins have been used to demonstrate the strong conservation of specific antigenic sites in all P. aeruginosa strains tested. In the case of one monoclonal antibody, specific for outer membrane lipoprotein H2, the antigenic site recognized by the antibody was also found to be conserved in all group 1 Pseudomonads. The implications of these monoclonal antibodies to bacterial taxonomy is discussed. Monoclonal antibodies against two separate conserved surface epitopes on outer membrane protein F were isolated and differentiated according to their reactions with 2 mercaptoethanol-reduced protein F and with proteolytic and cyanogen bromide peptide fragments of protein F. One of these protein F-specific monoclonal antibodies has been demonstrated to have immunotherapeutic potential.
| Language | English |
|---|---|
| Pages | 215-222 |
| Number of pages | 8 |
| Journal | Advances in experimental medicine and biology |
| Volume | 185 |
| Publication status | Published - 1 Dec 1985 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
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Monoclonal antibodies against bacterial outer membrane antigens. / Hancock, Robert; Mutharia, L. M.
In: Advances in experimental medicine and biology, Vol. 185, 01.12.1985, p. 215-222.Research output: Contribution to journal › Article
TY - JOUR
T1 - Monoclonal antibodies against bacterial outer membrane antigens.
AU - Hancock, Robert
AU - Mutharia, L. M.
PY - 1985/12/1
Y1 - 1985/12/1
N2 - Monoclonal antibodies have proved to be highly specific tools for defining the antigenic epitopes of Pseudomonas aeruginosa outer membrane macromolecules. In this article we have highlighted the use of monoclonal antibodies in the study of lipopolysaccharide heterogeneity and in particular have demonstrated that single monoclonal antibodies can recognize epitopes on lipid A which are conserved in all Gram negative bacteria tested. Monoclonal antibodies against P. aeruginosa outer membrane proteins have been used to demonstrate the strong conservation of specific antigenic sites in all P. aeruginosa strains tested. In the case of one monoclonal antibody, specific for outer membrane lipoprotein H2, the antigenic site recognized by the antibody was also found to be conserved in all group 1 Pseudomonads. The implications of these monoclonal antibodies to bacterial taxonomy is discussed. Monoclonal antibodies against two separate conserved surface epitopes on outer membrane protein F were isolated and differentiated according to their reactions with 2 mercaptoethanol-reduced protein F and with proteolytic and cyanogen bromide peptide fragments of protein F. One of these protein F-specific monoclonal antibodies has been demonstrated to have immunotherapeutic potential.
AB - Monoclonal antibodies have proved to be highly specific tools for defining the antigenic epitopes of Pseudomonas aeruginosa outer membrane macromolecules. In this article we have highlighted the use of monoclonal antibodies in the study of lipopolysaccharide heterogeneity and in particular have demonstrated that single monoclonal antibodies can recognize epitopes on lipid A which are conserved in all Gram negative bacteria tested. Monoclonal antibodies against P. aeruginosa outer membrane proteins have been used to demonstrate the strong conservation of specific antigenic sites in all P. aeruginosa strains tested. In the case of one monoclonal antibody, specific for outer membrane lipoprotein H2, the antigenic site recognized by the antibody was also found to be conserved in all group 1 Pseudomonads. The implications of these monoclonal antibodies to bacterial taxonomy is discussed. Monoclonal antibodies against two separate conserved surface epitopes on outer membrane protein F were isolated and differentiated according to their reactions with 2 mercaptoethanol-reduced protein F and with proteolytic and cyanogen bromide peptide fragments of protein F. One of these protein F-specific monoclonal antibodies has been demonstrated to have immunotherapeutic potential.
UR - http://www.scopus.com/inward/record.url?scp=0022333216&partnerID=8YFLogxK
M3 - Article
VL - 185
SP - 215
EP - 222
JO - Advances in Experimental Medicine and Biology
T2 - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -