Molecular methods in diagnosis and monitoring of haematological malignancies

David Yeung, Wendy T. Parker, Susan Branford

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The use of the polymerase chain reaction (PCR) was a revolutionary step in molecular biology, allowing for small amounts of genetic material to be amplified and studied. The advent of real-time PCR was a further refinement that led to reliable quantification of RNA and DNA. This allowed response monitoring and the detection of minimal residual disease, which proved to have important correlations with outcome in certain malignancies. The technology is indispensable for physicians and pathologists caring for oncology patients. In this article we will review the applications of molecular technology in the diagnosis and management of malignancies. Using chronic myeloid leukaemia (CML) as an example, technical aspects and clinical correlations will be discussed, with emphasis on the importance of quality assurance and standardisation to allow for comparability of results across laboratories. We will also examine emerging technologies that allow for high throughput and rapid turnaround of specimens and speculate how these would affect outcomes in future health care. The established and emerging molecular technologies have applications in many fields of oncology.

Original languageEnglish
Pages (from-to)566-579
Number of pages14
JournalPathology
Volume43
Issue number6
DOIs
Publication statusPublished - 2011
Externally publishedYes

Keywords

  • BCR-ABL1
  • Chronic myeloid leukaemia
  • Molecular techniques
  • Quantitative PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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