Methylation of CpG sites in exon 2 of the bcl-2 gene occurs in colorectal carcinoma

W. J. Babidge, L. M. Butler, M. A. Burton, P. A. Cowled

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14 Citations (Scopus)

Abstract

Background: Aberrant bcl-2 expression frequently occurs in colorectal carcinoma. The current study investigated if CpG sites in bcl-2 were methylated in colorectal carcinoma and if methylation correlated with loss of expression of bcl-2 mRNA. Methods: Methylation was assessed in 23 matched normal mucosae and colonic carcinomas by Southern blotting with methylation-sensitive enzymes. Expression of bcl-2 mRNA was assessed by Northern blotting. Results: A SacII site in exon 2 of the bcl-2 gene was methylated in 5 carcinomas, plus an adjacent HpaII sites in 1 tumour. SacII site in the bcl-2 promoter were not methylated. Elevated levels of bcl-2 mRNA were detected in 3 carcinomas, 5 showed decreased expression and 4 were unchanged. Conclusions: De novo methylation of CpG sites in exon 2 of the bcl-2 gene occurs during the development of colorectal carcinoma. However, there was no relationship between expression of bcl-2 mRNA and methylation of specific CpG sites.

Original languageEnglish
Pages (from-to)2809-2814
Number of pages6
JournalAnticancer Research
Volume21
Issue number4 A
Publication statusPublished - 24 Nov 2001

Keywords

  • Apoptosis
  • Bcl-2
  • Colorectal carcinoma
  • Gene expression
  • Methylation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Babidge, W. J., Butler, L. M., Burton, M. A., & Cowled, P. A. (2001). Methylation of CpG sites in exon 2 of the bcl-2 gene occurs in colorectal carcinoma. Anticancer Research, 21(4 A), 2809-2814.