Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads.

Ana V. Sánchez-Sánchez, Esther Camp-Dotlic, Antonio García-España, Aránzazu Leal-Tassias, José L. Mullor

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Oct4 is a crucial transcription factor for controlling pluripotency in embryonic stem cells and the epiblast of mouse embryos. We have characterized the expression pattern of medaka (Oryzias latipes) Ol-Oct4 during embryonic development and in the adult gonads. Genomic analysis showed that Ol-Oct4 is the ortholog of zebrafish spg/pou2. However, their expression patterns are not the same, suggesting that Oct4 may play different roles in zebrafish and medaka. Using specific antibodies for the Ol-Oct4 protein, we showed that Ol-Oct4 is also expressed in primordial germ cells, in the spermatogonia (male germ stem cells), and during different stages of oocyte development. These results suggest that Ol-Oct4 plays a post-embryonic role in the maturing gonads and gametes. The Ol-Oct4 mRNA and protein expression patterns are similar to those of mammalian Oct4 and introduce medaka fish as a valid model for the functional and evolutionary study of pluripotency genes in vivo.

LanguageEnglish
Pages672-679
Number of pages8
JournalDevelopmental dynamics : an official publication of the American Association of Anatomists
Volume239
Issue number2
DOIs
Publication statusPublished - 1 Jan 2010
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology

Cite this

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abstract = "Oct4 is a crucial transcription factor for controlling pluripotency in embryonic stem cells and the epiblast of mouse embryos. We have characterized the expression pattern of medaka (Oryzias latipes) Ol-Oct4 during embryonic development and in the adult gonads. Genomic analysis showed that Ol-Oct4 is the ortholog of zebrafish spg/pou2. However, their expression patterns are not the same, suggesting that Oct4 may play different roles in zebrafish and medaka. Using specific antibodies for the Ol-Oct4 protein, we showed that Ol-Oct4 is also expressed in primordial germ cells, in the spermatogonia (male germ stem cells), and during different stages of oocyte development. These results suggest that Ol-Oct4 plays a post-embryonic role in the maturing gonads and gametes. The Ol-Oct4 mRNA and protein expression patterns are similar to those of mammalian Oct4 and introduce medaka fish as a valid model for the functional and evolutionary study of pluripotency genes in vivo.",
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Medaka Oct4 is expressed during early embryo development, and in primordial germ cells and adult gonads. / Sánchez-Sánchez, Ana V.; Camp-Dotlic, Esther; García-España, Antonio; Leal-Tassias, Aránzazu; Mullor, José L.

In: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 239, No. 2, 01.01.2010, p. 672-679.

Research output: Contribution to journalArticle

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