Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI)

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Fast Ca 2+ -dependent inactivation (FCDI) is a safety mechanism limiting Ca 2+ entry through some types of Ca 2+ channels, including Ca 2+ release-activated Ca 2+ (CRAC) channels. This type of inactivation is caused by Ca 2+ , which passes through Ca 2+ channel and binds to a specific site within a short distance from the inner mouth of the pore, causing channel to shut. The main technique that is used to investigate FCDI is whole-cell patch clamping. Since the cloning of the molecular components of the CRAC channel, STIM1 and Orai1, FCDI of CRAC channel has been studied using HEK293T heterologous expression system. In this paper we describe a method of quantifying CRAC channel FCDI by using instantaneous tail currents.

LanguageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages167-173
Number of pages7
DOIs
Publication statusPublished - 1 Jan 2018

Publication series

NameMethods in Molecular Biology
Volume1843
ISSN (Print)1064-3745

Keywords

  • Boltzmann distribution
  • CRAC channels
  • Ca -dependent inactivation
  • Gating
  • Orai1
  • STIM1
  • Tail currents
  • Whole-cell patch clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Rychkov, G. (2018). Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI) In Methods in Molecular Biology (pp. 167-173). (Methods in Molecular Biology; Vol. 1843). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8704-7_14
Rychkov, Grigori. / Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI) Methods in Molecular Biology. Humana Press Inc., 2018. pp. 167-173 (Methods in Molecular Biology).
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Rychkov, G 2018, Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI) in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1843, Humana Press Inc., pp. 167-173. https://doi.org/10.1007/978-1-4939-8704-7_14

Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI) . / Rychkov, Grigori.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 167-173 (Methods in Molecular Biology; Vol. 1843).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Rychkov G. Measurement of the CRAC Channel Fast Ca 2+ -Dependent Inactivation (FCDI) In Methods in Molecular Biology. Humana Press Inc. 2018. p. 167-173. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8704-7_14