Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections

Paul Trim, Claire M. Henson, Jennie L. Avery, Andrew McEwen, Marten Snel, Emmanuelle Claude, Peter S. Marshall, Andrew West, Alessandra P. Princivalle, Malcolm R. Clench

Research output: Contribution to journalArticle

143 Citations (Scopus)

Abstract

During early-stage drug development, drug and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has a number of limitations, including the following: expensive synthesis of radiolabeled drugs and analyte specificity and identification. WBA only images the radiolabel. MALDI MSI has been shown previously to be advantageous for imaging the distribution of a range of drugs and metabolites in whole body sections. Ion mobility separation (IMS) adds a further separation step to imaging experiments; demonstrated here is MALDI-IMS-MS whole body imaging of rats dosed at 6 mg/kg IV with an anticancer drug, vinblastine and shown is the distribution of the precursor ion m/z 811.4 and several product ions including m/z 793, 751, 733, 719, 691, 649, 524, and 355. The distribution of vinblastine within the ventricles of the brain is also depicted. Clearly demonstrated in these data are the removal of interfering isobaric ions within the images of m/z 811.4 and also of the transition m/z 811-751, resulting in a higher confidence in the imaging data. Within this work, IMS has shown to be advantageous in both MS and MS/MS imaging experiments by separating vinblastine from an endogenous isobaric lipid.

LanguageEnglish
Pages8628-8634
Number of pages7
JournalAnalytical Chemistry
Volume80
Issue number22
DOIs
Publication statusPublished - 15 Nov 2008

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Trim, Paul ; Henson, Claire M. ; Avery, Jennie L. ; McEwen, Andrew ; Snel, Marten ; Claude, Emmanuelle ; Marshall, Peter S. ; West, Andrew ; Princivalle, Alessandra P. ; Clench, Malcolm R. / Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. In: Analytical Chemistry. 2008 ; Vol. 80, No. 22. pp. 8628-8634.
@article{1617ae196e234f71b5a18651a9554c79,
title = "Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections",
abstract = "During early-stage drug development, drug and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has a number of limitations, including the following: expensive synthesis of radiolabeled drugs and analyte specificity and identification. WBA only images the radiolabel. MALDI MSI has been shown previously to be advantageous for imaging the distribution of a range of drugs and metabolites in whole body sections. Ion mobility separation (IMS) adds a further separation step to imaging experiments; demonstrated here is MALDI-IMS-MS whole body imaging of rats dosed at 6 mg/kg IV with an anticancer drug, vinblastine and shown is the distribution of the precursor ion m/z 811.4 and several product ions including m/z 793, 751, 733, 719, 691, 649, 524, and 355. The distribution of vinblastine within the ventricles of the brain is also depicted. Clearly demonstrated in these data are the removal of interfering isobaric ions within the images of m/z 811.4 and also of the transition m/z 811-751, resulting in a higher confidence in the imaging data. Within this work, IMS has shown to be advantageous in both MS and MS/MS imaging experiments by separating vinblastine from an endogenous isobaric lipid.",
author = "Paul Trim and Henson, {Claire M.} and Avery, {Jennie L.} and Andrew McEwen and Marten Snel and Emmanuelle Claude and Marshall, {Peter S.} and Andrew West and Princivalle, {Alessandra P.} and Clench, {Malcolm R.}",
year = "2008",
month = "11",
day = "15",
doi = "10.1021/ac8015467",
language = "English",
volume = "80",
pages = "8628--8634",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "22",

}

Trim, P, Henson, CM, Avery, JL, McEwen, A, Snel, M, Claude, E, Marshall, PS, West, A, Princivalle, AP & Clench, MR 2008, 'Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections', Analytical Chemistry, vol. 80, no. 22, pp. 8628-8634. https://doi.org/10.1021/ac8015467

Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. / Trim, Paul; Henson, Claire M.; Avery, Jennie L.; McEwen, Andrew; Snel, Marten; Claude, Emmanuelle; Marshall, Peter S.; West, Andrew; Princivalle, Alessandra P.; Clench, Malcolm R.

In: Analytical Chemistry, Vol. 80, No. 22, 15.11.2008, p. 8628-8634.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections

AU - Trim, Paul

AU - Henson, Claire M.

AU - Avery, Jennie L.

AU - McEwen, Andrew

AU - Snel, Marten

AU - Claude, Emmanuelle

AU - Marshall, Peter S.

AU - West, Andrew

AU - Princivalle, Alessandra P.

AU - Clench, Malcolm R.

PY - 2008/11/15

Y1 - 2008/11/15

N2 - During early-stage drug development, drug and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has a number of limitations, including the following: expensive synthesis of radiolabeled drugs and analyte specificity and identification. WBA only images the radiolabel. MALDI MSI has been shown previously to be advantageous for imaging the distribution of a range of drugs and metabolites in whole body sections. Ion mobility separation (IMS) adds a further separation step to imaging experiments; demonstrated here is MALDI-IMS-MS whole body imaging of rats dosed at 6 mg/kg IV with an anticancer drug, vinblastine and shown is the distribution of the precursor ion m/z 811.4 and several product ions including m/z 793, 751, 733, 719, 691, 649, 524, and 355. The distribution of vinblastine within the ventricles of the brain is also depicted. Clearly demonstrated in these data are the removal of interfering isobaric ions within the images of m/z 811.4 and also of the transition m/z 811-751, resulting in a higher confidence in the imaging data. Within this work, IMS has shown to be advantageous in both MS and MS/MS imaging experiments by separating vinblastine from an endogenous isobaric lipid.

AB - During early-stage drug development, drug and metabolite distribution studies are carried out in animal tissues using a range of techniques, particularly whole body autoradiography (WBA). While widely employed, WBA has a number of limitations, including the following: expensive synthesis of radiolabeled drugs and analyte specificity and identification. WBA only images the radiolabel. MALDI MSI has been shown previously to be advantageous for imaging the distribution of a range of drugs and metabolites in whole body sections. Ion mobility separation (IMS) adds a further separation step to imaging experiments; demonstrated here is MALDI-IMS-MS whole body imaging of rats dosed at 6 mg/kg IV with an anticancer drug, vinblastine and shown is the distribution of the precursor ion m/z 811.4 and several product ions including m/z 793, 751, 733, 719, 691, 649, 524, and 355. The distribution of vinblastine within the ventricles of the brain is also depicted. Clearly demonstrated in these data are the removal of interfering isobaric ions within the images of m/z 811.4 and also of the transition m/z 811-751, resulting in a higher confidence in the imaging data. Within this work, IMS has shown to be advantageous in both MS and MS/MS imaging experiments by separating vinblastine from an endogenous isobaric lipid.

UR - http://www.scopus.com/inward/record.url?scp=56449126797&partnerID=8YFLogxK

U2 - 10.1021/ac8015467

DO - 10.1021/ac8015467

M3 - Article

VL - 80

SP - 8628

EP - 8634

JO - Analytical Chemistry

T2 - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 22

ER -