MALDI-ion mobility separation-mass spectrometry imaging of glucose-regulated protein 78 kDa (Grp78) in human formalin-fixed, paraffin-embedded pancreatic adenocarcinoma tissue sections

Marie Claude Djidja, Emmanuelle Claude, Marten F. Snel, Peter Scriven, Simona Francese, Vikki Carolan, Malcolm R. Clench

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

MALDI-mass spectrometry imaging (MALDI-MSI) is a technique that allows proteomic information, that is, the spatial distribution and identification of proteins, to be obtained directly from tissue sections. The use of in situ enzymatic digestion as a sample pretreatment prior to MALDI-MSI analysis has been found to be useful for retrieving protein identification directly from formalin-fixed, paraffin-embedded (ffpe) tissue sections. Here, an improved method for the study of the distribution and the identification of peptides obtained after in situ digestion of fppe pancreatic tumor tissue sections by using MALDI-mass spectrometry imaging coupled with ion mobility separation (IMS) is described. MALDI-IMS-MS images of peptide obtained from pancreatic tumor tissue sections allowed the localization of tumor regions within the tissue section, while minimizing the peak interferences which were observed with conventional MALDI-TOF MSI. The use of ion mobility separation coupled with MALDI-MSI improved the selectivity and specificity of the method and, hence, enabled both the localization and in situ identification of glucose regulated protein 78 kDa (Grp78), a tumor biomarker, within pancreatic tumor tissue sections. These findings were validated using immunohistochemical staining.

LanguageEnglish
Pages4876-4884
Number of pages9
JournalJournal of Proteome Research
Volume8
Issue number10
DOIs
Publication statusPublished - 2009

Keywords

  • Formalin-fixed, paraffin-embedded
  • Grp78
  • Imaging
  • Ion mobility separation
  • MALDI
  • Pancreatic cancer

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Djidja, Marie Claude ; Claude, Emmanuelle ; Snel, Marten F. ; Scriven, Peter ; Francese, Simona ; Carolan, Vikki ; Clench, Malcolm R. / MALDI-ion mobility separation-mass spectrometry imaging of glucose-regulated protein 78 kDa (Grp78) in human formalin-fixed, paraffin-embedded pancreatic adenocarcinoma tissue sections. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 10. pp. 4876-4884.
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abstract = "MALDI-mass spectrometry imaging (MALDI-MSI) is a technique that allows proteomic information, that is, the spatial distribution and identification of proteins, to be obtained directly from tissue sections. The use of in situ enzymatic digestion as a sample pretreatment prior to MALDI-MSI analysis has been found to be useful for retrieving protein identification directly from formalin-fixed, paraffin-embedded (ffpe) tissue sections. Here, an improved method for the study of the distribution and the identification of peptides obtained after in situ digestion of fppe pancreatic tumor tissue sections by using MALDI-mass spectrometry imaging coupled with ion mobility separation (IMS) is described. MALDI-IMS-MS images of peptide obtained from pancreatic tumor tissue sections allowed the localization of tumor regions within the tissue section, while minimizing the peak interferences which were observed with conventional MALDI-TOF MSI. The use of ion mobility separation coupled with MALDI-MSI improved the selectivity and specificity of the method and, hence, enabled both the localization and in situ identification of glucose regulated protein 78 kDa (Grp78), a tumor biomarker, within pancreatic tumor tissue sections. These findings were validated using immunohistochemical staining.",
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MALDI-ion mobility separation-mass spectrometry imaging of glucose-regulated protein 78 kDa (Grp78) in human formalin-fixed, paraffin-embedded pancreatic adenocarcinoma tissue sections. / Djidja, Marie Claude; Claude, Emmanuelle; Snel, Marten F.; Scriven, Peter; Francese, Simona; Carolan, Vikki; Clench, Malcolm R.

In: Journal of Proteome Research, Vol. 8, No. 10, 2009, p. 4876-4884.

Research output: Contribution to journalArticle

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AU - Scriven, Peter

AU - Francese, Simona

AU - Carolan, Vikki

AU - Clench, Malcolm R.

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