Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells

V. Haridas, Sandhya Sadanandan, Pierre Yves Collart-Dutilleul, Stan Gronthos, Nicolas H. Voelcker

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysineappended diacetylenes generated a variety of polymers (P1. P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys Nα-NH2and Lys Nε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with Nε-Boc and Nα-Fmoc or more hydrophilic polymer P5 containing free Nεand Nαamino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.

LanguageEnglish
Pages582-590
Number of pages9
JournalBiomacromolecules
Volume15
Issue number2
DOIs
Publication statusPublished - 10 Feb 2014
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Haridas, V., Sadanandan, S., Collart-Dutilleul, P. Y., Gronthos, S., & Voelcker, N. H. (2014). Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells. Biomacromolecules, 15(2), 582-590. https://doi.org/10.1021/bm4015655
Haridas, V. ; Sadanandan, Sandhya ; Collart-Dutilleul, Pierre Yves ; Gronthos, Stan ; Voelcker, Nicolas H. / Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells. In: Biomacromolecules. 2014 ; Vol. 15, No. 2. pp. 582-590.
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Haridas, V, Sadanandan, S, Collart-Dutilleul, PY, Gronthos, S & Voelcker, NH 2014, 'Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells', Biomacromolecules, vol. 15, no. 2, pp. 582-590. https://doi.org/10.1021/bm4015655

Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells. / Haridas, V.; Sadanandan, Sandhya; Collart-Dutilleul, Pierre Yves; Gronthos, Stan; Voelcker, Nicolas H.

In: Biomacromolecules, Vol. 15, No. 2, 10.02.2014, p. 582-590.

Research output: Contribution to journalArticle

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Haridas V, Sadanandan S, Collart-Dutilleul PY, Gronthos S, Voelcker NH. Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells. Biomacromolecules. 2014 Feb 10;15(2):582-590. https://doi.org/10.1021/bm4015655