Abstract
We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysineappended diacetylenes generated a variety of polymers (P1. P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys Nα-NH2and Lys Nε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with Nε-Boc and Nα-Fmoc or more hydrophilic polymer P5 containing free Nεand Nαamino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
| Language | English |
|---|---|
| Pages | 582-590 |
| Number of pages | 9 |
| Journal | Biomacromolecules |
| Volume | 15 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 10 Feb 2014 |
| Externally published | Yes |
ASJC Scopus subject areas
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry
Cite this
}
Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells. / Haridas, V.; Sadanandan, Sandhya; Collart-Dutilleul, Pierre Yves; Gronthos, Stan; Voelcker, Nicolas H.
In: Biomacromolecules, Vol. 15, No. 2, 10.02.2014, p. 582-590.Research output: Contribution to journal › Article
TY - JOUR
T1 - Lysine-appended polydiacetylene scaffolds for human mesenchymal stem cells
AU - Haridas, V.
AU - Sadanandan, Sandhya
AU - Collart-Dutilleul, Pierre Yves
AU - Gronthos, Stan
AU - Voelcker, Nicolas H.
PY - 2014/2/10
Y1 - 2014/2/10
N2 - We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysineappended diacetylenes generated a variety of polymers (P1. P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys Nα-NH2and Lys Nε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with Nε-Boc and Nα-Fmoc or more hydrophilic polymer P5 containing free Nεand Nαamino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
AB - We report on the self-assembly based fabrication of fibrous polymers for tissue engineering applications. Directed self-assembly followed by polymerization of lysineappended diacetylenes generated a variety of polymers (P1. P5) with distinct chemical properties. The self-assembly along with the conjugated double and triple bonds and rigid geometry of diacetylene backbone imposed a nanofibrous morphology on the resulting polymers. Chemical properties including wettability of the polymers were tuned by using lysine (Lys) with orthogonal protecting groups (Boc and Fmoc). These Lys-appended polydiacetylene scaffolds were compared in terms of their efficiency toward human mesenchymal stem cells adhesion and spreading. Interestingly, polymer P4 containing Lys Nα-NH2and Lys Nε-Boc with balanced wettability supported cell adhesion better than the more hydrophobic polymer P2 with Nε-Boc and Nα-Fmoc or more hydrophilic polymer P5 containing free Nεand Nαamino groups. The molecular level control in the fabrication of nanofibrous polymers compared with other existing methods for the generation of fibrous polymers is the hallmark of this work.
UR - http://www.scopus.com/inward/record.url?scp=84896788210&partnerID=8YFLogxK
U2 - 10.1021/bm4015655
DO - 10.1021/bm4015655
M3 - Article
VL - 15
SP - 582
EP - 590
JO - Biomacromolecules
T2 - Biomacromolecules
JF - Biomacromolecules
SN - 1525-7797
IS - 2
ER -