LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages

Flor Torres-Juarez, Albertina Cardenas-Vargas, Alejandra Montoya-Rosales, Irma González-Curiel, Mariana H. Garcia-Hernandez, Jose A. Enciso-Moreno, Robert E.W. Hancock, Bruno Rivas-Santiago

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33 Citations (Scopus)

Abstract

Tuberculosis is one of the most important infectious diseases worldwide. The susceptibility to this disease depends to a great extent on the innate immune response against mycobacteria. Host defense peptides (HDP) are one of the first barriers to counteract infection. Cathelicidin (LL-37) is an HDP that has many immunomodulatory effects besides its weak antimicrobial activity. Despite advances in the study of the innate immune response in tuberculosis, the immunological role of LL-37 during M. tuberculosis infection has not been clarified. Monocyte-derived macrophages were infected with M. tuberculosis strain H37Rv and then treated with 1, 5, or 15 μg/ml of exogenous LL-37 for 4, 8, and 24 h. Exogenous LL-37 decreased tumor necrosis factor alpha (TNF-α) and interleukin-17 (IL-17) while inducing anti-inflammatory IL-10 and transforming growth factor β (TGF-β) production. Interestingly, the decreased production of anti-inflammatory cytokines did not reduce antimycobacterial activity. These results are consistent with the concept that LL-37 can modulate the expression of cytokines during mycobacterial infection and this activity was independent of the P2X7 receptor. Thus, LL-37 modulates the response of macrophages during infection, controlling the expression of proinflammatory and anti-inflammatory cytokines.

Original languageEnglish
Pages (from-to)4495-4503
Number of pages9
JournalInfection and Immunity
Volume83
Issue number12
DOIs
Publication statusPublished - 1 Jan 2015

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Torres-Juarez, F., Cardenas-Vargas, A., Montoya-Rosales, A., González-Curiel, I., Garcia-Hernandez, M. H., Enciso-Moreno, J. A., ... Rivas-Santiago, B. (2015). LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages. Infection and Immunity, 83(12), 4495-4503. https://doi.org/10.1128/IAI.00936-15