LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages

Flor Torres-Juarez, Albertina Cardenas-Vargas, Alejandra Montoya-Rosales, Irma González-Curiel, Mariana H. Garcia-Hernandez, Jose A. Enciso-Moreno, Robert Hancock, Bruno Rivas-Santiago

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Tuberculosis is one of the most important infectious diseases worldwide. The susceptibility to this disease depends to a great extent on the innate immune response against mycobacteria. Host defense peptides (HDP) are one of the first barriers to counteract infection. Cathelicidin (LL-37) is an HDP that has many immunomodulatory effects besides its weak antimicrobial activity. Despite advances in the study of the innate immune response in tuberculosis, the immunological role of LL-37 during M. tuberculosis infection has not been clarified. Monocyte-derived macrophages were infected with M. tuberculosis strain H37Rv and then treated with 1, 5, or 15 μg/ml of exogenous LL-37 for 4, 8, and 24 h. Exogenous LL-37 decreased tumor necrosis factor alpha (TNF-α) and interleukin-17 (IL-17) while inducing anti-inflammatory IL-10 and transforming growth factor β (TGF-β) production. Interestingly, the decreased production of anti-inflammatory cytokines did not reduce antimycobacterial activity. These results are consistent with the concept that LL-37 can modulate the expression of cytokines during mycobacterial infection and this activity was independent of the P2X7 receptor. Thus, LL-37 modulates the response of macrophages during infection, controlling the expression of proinflammatory and anti-inflammatory cytokines.

LanguageEnglish
Pages4495-4503
Number of pages9
JournalInfection and Immunity
Volume83
Issue number12
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Torres-Juarez, F., Cardenas-Vargas, A., Montoya-Rosales, A., González-Curiel, I., Garcia-Hernandez, M. H., Enciso-Moreno, J. A., ... Rivas-Santiago, B. (2015). LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages. Infection and Immunity, 83(12), 4495-4503. https://doi.org/10.1128/IAI.00936-15
Torres-Juarez, Flor ; Cardenas-Vargas, Albertina ; Montoya-Rosales, Alejandra ; González-Curiel, Irma ; Garcia-Hernandez, Mariana H. ; Enciso-Moreno, Jose A. ; Hancock, Robert ; Rivas-Santiago, Bruno. / LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages. In: Infection and Immunity. 2015 ; Vol. 83, No. 12. pp. 4495-4503.
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Torres-Juarez, F, Cardenas-Vargas, A, Montoya-Rosales, A, González-Curiel, I, Garcia-Hernandez, MH, Enciso-Moreno, JA, Hancock, R & Rivas-Santiago, B 2015, 'LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages', Infection and Immunity, vol. 83, no. 12, pp. 4495-4503. https://doi.org/10.1128/IAI.00936-15

LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages. / Torres-Juarez, Flor; Cardenas-Vargas, Albertina; Montoya-Rosales, Alejandra; González-Curiel, Irma; Garcia-Hernandez, Mariana H.; Enciso-Moreno, Jose A.; Hancock, Robert; Rivas-Santiago, Bruno.

In: Infection and Immunity, Vol. 83, No. 12, 01.01.2015, p. 4495-4503.

Research output: Contribution to journalArticle

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AU - Cardenas-Vargas, Albertina

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Torres-Juarez F, Cardenas-Vargas A, Montoya-Rosales A, González-Curiel I, Garcia-Hernandez MH, Enciso-Moreno JA et al. LL-37 immunomodulatory activity during Mycobacterium tuberculosis infection in macrophages. Infection and Immunity. 2015 Jan 1;83(12):4495-4503. https://doi.org/10.1128/IAI.00936-15