Liquid chromatography/electrospray ionisation-tandem mass spectrometry quantification of GM2 gangliosides in human peripheral cells and plasma

Maria Fuller, Stephen Duplock, Leanne K. Hein, Brigitte A. Rigat, Don J. Mahuran

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

GM2 gangliosidosis is a group of inherited neurodegenerative disorders resulting primarily from the excessive accumulation of GM2 gangliosides (GM2) in neuronal cells. As biomarkers for categorising patients and monitoring the effectiveness of developing therapies are lacking for this group of disorders, we sought to develop methodology to quantify G M2 levels in more readily attainable patient samples such as plasma, leukocytes, and cultured skin fibroblasts. Following organic extraction, gangliosides were partitioned into the aqueous phase and isolated using C18 solid-phase extraction columns. Relative quantification of three species of GM2 was achieved using LC/ESI-MS/MS with d35GM1 18:1/18:0 as an internal standard. The assay was linear over the biological range, and all GM2 gangliosidosis patients were demarcated from controls by elevated GM2 in cultured skin fibroblast extracts. However, in leukocytes only some molecular species could be used for differentiation and in plasma only one was informative. A reduction in G M2 was easily detected in patient skin fibroblasts after a short treatment with media from normal cells enriched in secreted β- hexosaminidase. This method may show promise for measuring the effectiveness of experimental therapies for GM2 gangliosidosis by allowing quantification of a reduction in the primary storage burden.

Original languageEnglish
Pages (from-to)20-26
Number of pages7
JournalAnalytical Biochemistry
Volume458
DOIs
Publication statusPublished - 1 Aug 2014

Keywords

  • Ganglioside
  • Mass spectrometry
  • Sandhoff disease
  • Tay-Sachs disease

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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