Lineage differentiation of mesenchymal stem cells from dental pulp, Apical Papilla, and periodontal ligament

Kentaro Akiyama, Chider Chen, Stan Gronthos, Songtao Shi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

28 Citations (Scopus)


Recently, a variety of mesenchymal stem cells (MSCs), including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and mesenchymal stem cells derived from human gingival, were isolated from orofacial and dental tissues. However, it is unknown whether these orofacial stem cells are derived from mesoderm or neural crest cell. In order to encourage orofacial MSC investigation, we provide detailed protocols for assessing lineage A differentiation of orofacial MSCs.

Original languageEnglish
Title of host publicationOdontogenesis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages11
ISBN (Print)9781617798597
Publication statusPublished or Issued - 1 Jan 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Dental pulp stem cells
  • Lineage differentiation
  • Mesenchymal stem cells
  • Periodontal ligament stem cells
  • Stem cell from apical papilla
  • Stem cells from human exfoliated deciduous teeth

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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