LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera

George C. Gussenhoven, Menno A W G Van Der Hoorn, Marga G A Goris, Wiepko J. Terpstra, Rudy A. Hartskeerl, Ben W. Mol, Cor W. Van Ingen, Henk L. Smits

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

We studied a dipstick assay for the detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human serum samples. A high degree of concordance was observed between the results of the dipstick assay and an IgM enzyme-linked immunosorbent assay (ELISA). Application of the dipstick assay for the detection of acute leptospirosis enabled the accurate identification, early in the disease, of a high proportion of the cases of leptospirosis. Analysis of a second serum sample is recommended, in order to determine seroconversion or increased staining intensity. All serum samples from the patients who were confirmed to be positive for leptospirosis by either a positive microscopic agglutination test or a positive culture but were found to be negative by the dipstick assay were also judged to be negative by the IgM ELISA or revealed borderline titers by the IgM ELISA. Some cross- reactivity was observed for sera from patients with diseases other than leptospirosis, and this should be taken into account in the interpretation of test results. The dipstick assay is easy to perform, can be performed quickly, and requires no electricity or special equipment, and the assay components, a dipstick and a staining reagent, can be stored for a prolonged period without a loss of reactivity, even at elevated temperatures.

LanguageEnglish
Pages92-97
Number of pages6
JournalJournal of Clinical Microbiology
Volume35
Issue number1
Publication statusPublished - Jan 1997
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Gussenhoven, G. C., Van Der Hoorn, M. A. W. G., Goris, M. G. A., Terpstra, W. J., Hartskeerl, R. A., Mol, B. W., ... Smits, H. L. (1997). LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. Journal of Clinical Microbiology, 35(1), 92-97.
Gussenhoven, George C. ; Van Der Hoorn, Menno A W G ; Goris, Marga G A ; Terpstra, Wiepko J. ; Hartskeerl, Rudy A. ; Mol, Ben W. ; Van Ingen, Cor W. ; Smits, Henk L. / LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. In: Journal of Clinical Microbiology. 1997 ; Vol. 35, No. 1. pp. 92-97.
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Gussenhoven, GC, Van Der Hoorn, MAWG, Goris, MGA, Terpstra, WJ, Hartskeerl, RA, Mol, BW, Van Ingen, CW & Smits, HL 1997, 'LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera', Journal of Clinical Microbiology, vol. 35, no. 1, pp. 92-97.

LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. / Gussenhoven, George C.; Van Der Hoorn, Menno A W G; Goris, Marga G A; Terpstra, Wiepko J.; Hartskeerl, Rudy A.; Mol, Ben W.; Van Ingen, Cor W.; Smits, Henk L.

In: Journal of Clinical Microbiology, Vol. 35, No. 1, 01.1997, p. 92-97.

Research output: Contribution to journalArticle

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Gussenhoven GC, Van Der Hoorn MAWG, Goris MGA, Terpstra WJ, Hartskeerl RA, Mol BW et al. LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. Journal of Clinical Microbiology. 1997 Jan;35(1):92-97.