Interleukin-22 promotes phagolysosomal fusion to induce protection against Salmonella enterica Typhimurium in human epithelial cells

Jessica L. Forbester, Emily A. Lees, David Goulding, Sally Forrest, Amy Yeung, Anneliese Speak, Simon Clare, Eve L. Coomber, Subhankar Mukhopadhyay, Judith Kraiczy, Fernanda Schreiber, Trevor D. Lawley, Robert E.W. Hancock, Holm H. Uhlig, Matthias Zilbauer, Fiona Powrie, Gordon Dougan

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


Intestinal epithelial cells (IECs) play a key role in regulating immune responses and controlling infection. However, the direct role of IECs in restricting pathogens remains incompletely understood. Here, we provide evidence that IL-22 primed intestinal organoids derived from healthy human induced pluripotent stem cells (hIPSCs) to restrict Salmonella enterica serovar Typhimurium SL1344 infection. A combination of transcriptomics, bacterial invasion assays, and imaging suggests that IL-22–induced antimicrobial activity is driven by increased phagolysosomal fusion in IL-22–pretreated cells. The antimicrobial phenotype was absent in hIPSCs derived from a patient harboring a homozygous mutation in the IL10RB gene that inactivates the IL-22 receptor but was restored by genetically complementing the IL10RB deficiency. This study highlights a mechanism through which the IL-22 pathway facilitates the human intestinal epithelium to control microbial infection.

Original languageEnglish
Pages (from-to)10118-10123
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number40
Publication statusPublished or Issued - 2 Oct 2018


  • Interleukin-22
  • Intestinal organoids
  • Salmonella

ASJC Scopus subject areas

  • General

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