Interleukin 1 receptor antagonist gene expression in rat pituitary in the systemic inflammatory response syndrome: Pathophysiological implications

J. Licinio, M. L. Wong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The systemic inflammatory response syndrome (SIRS) is characterized by endothelial cell activation causing a generalized inflammatory response and cytokine-mediated pathophysiological alterations. The pathophysiology of SIRS involves changes in the functioning of several endocrine glands, including the pituitary. SIRS-induced alterations in pituitary function include activation of the hypothalamic-pituitary adrenal (HPA) axis causing hypercortisolemia, the euthyroid sick syndrome, and cessation of reproductive function. These changes are in part mediated by cytokines, such as tumor necrosis factor α (TNF-α) and interleukin 1 (IL-1). IL-1 function depends on the local ratio of bioactive IL-1 and IL-1 receptor antagonist. Here we review our studies on IL-1β and IL-1ra mRNA levels in the pituitary in a rat model of SIRS. We have found that IL-1β mRNA peaked at 2 h after lipopolysaccharide (LPS) administration, increasing twelvefold over control values in the posterior pituitary and fivefold over controls in the anterior pituitary. IL-1ra mRNA levels peaked at 6 h post-LPS administration, later than those of IL-1β mRNA. IL-1ra mRNA levels increased tenfold in the anterior pituitary, but were induced only threefold in the posterior pituitary. IL-1ra gene expression is profoundly induced in the pituitary in vivo during systemic inflammation and its induction follows that of IL-1β, but it is differentially regulated and tissue-specific, occurring predominantly in the anterior pituitary. Future studies of the effects of IL-1 in the pituitary should take into account the local levels of IL-1ra.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalMolecular Psychiatry
Volume2
Issue number2
DOIs
Publication statusPublished - 1 Jan 1997

Keywords

  • In situ hybridization
  • Interleukin 1 receptor antagonist
  • Interleukin 1β
  • Lipopolysaccharide
  • Pituitary gland
  • Sepsis
  • Systemic inflammatory response syndrome

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

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