In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments

Farzana Kastury, Euan Smith, Emmanuel Doelsch, Enzo Lombi, Martin Donnelley, Patricia L. Cmielewski, David W. Parsons, Kirk G. Scheckel, David Paterson, Martin D. De Jonge, Carina Herde, Albert L. Juhasz

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Abstract

This study compared lead (Pb) immobilization efficacies in mining/smelting impacted soil using phosphate and iron amendments via ingestion and inhalation pathways using in vitro and in vivo assays, in conjunction with investigating the dynamics of dust particles in the lungs and gastro-intestinal tract via X-ray fluorescence (XRF) microscopy. Phosphate amendments [phosphoric acid (PA), hydroxyapatite, monoammonium phosphate (MAP), triple super phosphate (TSP), and bone meal biochar] and hematite were applied at a molar ratio of Pb:Fe/P = 1:5. Pb phosphate formation was investigated in the soil/post-in vitro bioaccessibility (IVBA) residuals and in mouse lung via extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structures (XANES) spectroscopy, respectively. EXAFS analysis revealed that anglesite was the dominant phase in the ingestible (<250 μm) and inhalable (<10 μm) particle fractions. Pb IVBA was significantly reduced (p < 0.05) by phosphate amendments in the <250 μm fraction (solubility bioaccessibility research consortium assay) and by PA, MAP, and TSP in the <10 μm fraction (inhalation-ingestion bioaccessibility assay). A 21.1% reduction in Pb RBA (<250 μm fraction) and 56.4% reduction in blood Pb concentration (<10 μm fraction) were observed via the ingestion and inhalation pathways, respectively. XRF microscopy detected Pb in the stomach within 4 h, presumably via mucociliary clearance.

LanguageEnglish
JournalEnvironmental Science and Technology
DOIs
Publication statusPublished - 29 Jul 2019

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Kastury, Farzana ; Smith, Euan ; Doelsch, Emmanuel ; Lombi, Enzo ; Donnelley, Martin ; Cmielewski, Patricia L. ; Parsons, David W. ; Scheckel, Kirk G. ; Paterson, David ; De Jonge, Martin D. ; Herde, Carina ; Juhasz, Albert L. / In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments. In: Environmental Science and Technology. 2019.
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abstract = "This study compared lead (Pb) immobilization efficacies in mining/smelting impacted soil using phosphate and iron amendments via ingestion and inhalation pathways using in vitro and in vivo assays, in conjunction with investigating the dynamics of dust particles in the lungs and gastro-intestinal tract via X-ray fluorescence (XRF) microscopy. Phosphate amendments [phosphoric acid (PA), hydroxyapatite, monoammonium phosphate (MAP), triple super phosphate (TSP), and bone meal biochar] and hematite were applied at a molar ratio of Pb:Fe/P = 1:5. Pb phosphate formation was investigated in the soil/post-in vitro bioaccessibility (IVBA) residuals and in mouse lung via extended X-ray absorption fine structure (EXAFS) and X-ray absorption near edge structures (XANES) spectroscopy, respectively. EXAFS analysis revealed that anglesite was the dominant phase in the ingestible (<250 μm) and inhalable (<10 μm) particle fractions. Pb IVBA was significantly reduced (p < 0.05) by phosphate amendments in the <250 μm fraction (solubility bioaccessibility research consortium assay) and by PA, MAP, and TSP in the <10 μm fraction (inhalation-ingestion bioaccessibility assay). A 21.1{\%} reduction in Pb RBA (<250 μm fraction) and 56.4{\%} reduction in blood Pb concentration (<10 μm fraction) were observed via the ingestion and inhalation pathways, respectively. XRF microscopy detected Pb in the stomach within 4 h, presumably via mucociliary clearance.",
author = "Farzana Kastury and Euan Smith and Emmanuel Doelsch and Enzo Lombi and Martin Donnelley and Cmielewski, {Patricia L.} and Parsons, {David W.} and Scheckel, {Kirk G.} and David Paterson and {De Jonge}, {Martin D.} and Carina Herde and Juhasz, {Albert L.}",
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month = "7",
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In Vitro, in Vivo, and Spectroscopic Assessment of Lead Exposure Reduction via Ingestion and Inhalation Pathways Using Phosphate and Iron Amendments. / Kastury, Farzana; Smith, Euan; Doelsch, Emmanuel; Lombi, Enzo; Donnelley, Martin; Cmielewski, Patricia L.; Parsons, David W.; Scheckel, Kirk G.; Paterson, David; De Jonge, Martin D.; Herde, Carina; Juhasz, Albert L.

In: Environmental Science and Technology, 29.07.2019.

Research output: Contribution to journalArticle

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AU - Kastury, Farzana

AU - Smith, Euan

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AU - Lombi, Enzo

AU - Donnelley, Martin

AU - Cmielewski, Patricia L.

AU - Parsons, David W.

AU - Scheckel, Kirk G.

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AU - Herde, Carina

AU - Juhasz, Albert L.

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