In depth analysis of the Sox4 gene locus that consists of sense and natural antisense transcripts

King Hwa Ling, Peter J. Brautigan, Sarah Moore, Rachel Fraser, Melody Pui Yee Leong, Jia Wen Leong, Shahidee Zainal Abidin, Han Chung Lee, Pike See Cheah, Joy M. Raison, Milena Babic, Young Kyung Lee, Tasman Daish, Deidre M. Mattiske, Jeffrey R. Mann, David L. Adelson, Paul Q. Thomas, Christopher N. Hahn, Hamish S. Scott

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

SRY (Sex Determining Region Y)-Box 4 or Sox4 is an important regulator of the pan-neuronal gene expression during post-mitotic cell differentiation within the mammalian brain. Sox4 gene locus has been previously characterized with multiple sense and overlapping natural antisense transcripts [1,2]. Here we provide accompanying data on various analyses performed and described in Ling et al. [2]. The data include a detail description of various features found at Sox4 gene locus, additional experimental data derived from RNA-Fluorescence in situ Hybridization (RNA-FISH), Western blotting, strand-specific reverse-transcription quantitative polymerase chain reaction (RT-qPCR), gain-of-function and in situ hybridization (ISH) experiments. All the additional data provided here support the existence of an endogenous small interfering- or PIWI interacting-like small RNA known as Sox4_sir3, which origin was found within the overlapping region consisting of a sense and a natural antisense transcript known as Sox4ot1.

Original languageEnglish
Pages (from-to)282-290
Number of pages9
JournalData in Brief
Volume7
DOIs
Publication statusPublished or Issued - 1 Jun 2016
Externally publishedYes

Keywords

  • Brain development
  • Endogenous siRNA
  • Natural antisense transcripts

ASJC Scopus subject areas

  • General

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