Implanted adult human dental pulp stem cells induce endogenous axon guidance

Agnieszka Arthur, Songtao Shi, Andrew C W Zannettino, Nobutaka Fujii, Stan Gronthos, Simon A. Koblar

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The human central nervous system has limited capacity for regeneration. Stem cell-based therapies may overcome this through cellular mechanisms of neural replacement and/or through molecular mechanisms, whereby secreted factors induce change in the host tissue. To investigate these mechanisms, we used a readily accessible human cell population, dental pulp progenitor/stem cells (DPSCs) that can differentiate into functionally active neurons given the appropriate environmental cues. We hypothesized that implanted DPSCs secrete factors that coordinate axon guidance within a receptive host nervous system. An avian embryonic model system was adapted to investigate axon guidance in vivo after transplantation of adult human DPSCs. Chemoattraction of avian trigeminal ganglion axons toward implanted DPSCs was mediated via the chemokine, CXCL12, also known as stromal cell-derived factor-1, and its receptor, CXCR4. These findings provide the first direct evidence that DPSCs may induce neuroplasticity within a receptive host nervous system.

Original languageEnglish
Pages (from-to)2229-2237
Number of pages9
JournalStem Cells
Volume27
Issue number9
DOIs
Publication statusPublished - 1 Sep 2009
Externally publishedYes

Keywords

  • Mesenchymal/neural crest stem cells
  • Neuroplasticity SDF-1
  • Stem cell transplantation

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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