Immunomodulatory properties of stem cells from human exfoliated deciduous teeth

Takayoshi Yamaza, Akiyama Kentaro, Chider Chen, Yi Liu, Yufang Shi, Stan Gronthos, Songlin Wang, Songtao Shi

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

Introduction. Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a population of postnatal stem cells capable of differentiating into osteogenic and odontogenic cells, adipogenic cells, and neural cells. Herein we have characterized mesenchymal stem cell properties of SHED in comparison to human bone marrow mesenchymal stem cells (BMMSCs). Methods. We used in vitro stem cell analysis approaches, including flow cytometry, inductive differentiation, telomerase activity, and Western blot analysis to assess multipotent differentiation of SHED and in vivo implantation to assess tissue regeneration of SHED. In addition, we utilized systemic SHED transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: We found that SHED are capable of differentiating into osteogenic and adipogenic cells, expressing mesenchymal surface molecules (STRO-1, CD146, SSEA4, CD73, CD105, and CD166), and activating multiple signaling pathways, including TGF, ERK, Akt, Wnt, and PDGF. Recently, BMMSCs were shown to possess an immunomodulatory function that leads to successful therapies for immune diseases. We examined the immunomodulatory properties of SHED in comparison to BMMSCs and found that SHED had significant effects on inhibiting T helper 17 (Th17) cells in vitro. Moreover, we found that SHED transplantation is capable of effectively reversing SLE-associated disorders in MRL/lpr mice. At the cellular level, SHED transplantation elevated the ratio of regulatory T cells (Tregs) via Th17 cells. Conclusions: These data suggest that SHED are an accessible and feasible mesenchymal stem cell source for treating immune disorders like SLE.

LanguageEnglish
Article number5
JournalStem Cell Research and Therapy
Volume1
Issue number1
DOIs
Publication statusPublished - 1 Dec 2010

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)
  • Cell Biology

Cite this

Yamaza, Takayoshi ; Kentaro, Akiyama ; Chen, Chider ; Liu, Yi ; Shi, Yufang ; Gronthos, Stan ; Wang, Songlin ; Shi, Songtao. / Immunomodulatory properties of stem cells from human exfoliated deciduous teeth. In: Stem Cell Research and Therapy. 2010 ; Vol. 1, No. 1.
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Immunomodulatory properties of stem cells from human exfoliated deciduous teeth. / Yamaza, Takayoshi; Kentaro, Akiyama; Chen, Chider; Liu, Yi; Shi, Yufang; Gronthos, Stan; Wang, Songlin; Shi, Songtao.

In: Stem Cell Research and Therapy, Vol. 1, No. 1, 5, 01.12.2010.

Research output: Contribution to journalArticle

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AU - Yamaza, Takayoshi

AU - Kentaro, Akiyama

AU - Chen, Chider

AU - Liu, Yi

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AU - Gronthos, Stan

AU - Wang, Songlin

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N2 - Introduction. Stem cells from human exfoliated deciduous teeth (SHED) have been identified as a population of postnatal stem cells capable of differentiating into osteogenic and odontogenic cells, adipogenic cells, and neural cells. Herein we have characterized mesenchymal stem cell properties of SHED in comparison to human bone marrow mesenchymal stem cells (BMMSCs). Methods. We used in vitro stem cell analysis approaches, including flow cytometry, inductive differentiation, telomerase activity, and Western blot analysis to assess multipotent differentiation of SHED and in vivo implantation to assess tissue regeneration of SHED. In addition, we utilized systemic SHED transplantation to treat systemic lupus erythematosus (SLE)-like MRL/lpr mice. Results: We found that SHED are capable of differentiating into osteogenic and adipogenic cells, expressing mesenchymal surface molecules (STRO-1, CD146, SSEA4, CD73, CD105, and CD166), and activating multiple signaling pathways, including TGF, ERK, Akt, Wnt, and PDGF. Recently, BMMSCs were shown to possess an immunomodulatory function that leads to successful therapies for immune diseases. We examined the immunomodulatory properties of SHED in comparison to BMMSCs and found that SHED had significant effects on inhibiting T helper 17 (Th17) cells in vitro. Moreover, we found that SHED transplantation is capable of effectively reversing SLE-associated disorders in MRL/lpr mice. At the cellular level, SHED transplantation elevated the ratio of regulatory T cells (Tregs) via Th17 cells. Conclusions: These data suggest that SHED are an accessible and feasible mesenchymal stem cell source for treating immune disorders like SLE.

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