Immunomodulatory Properties of Induced Pluripotent Stem Cell-Derived Mesenchymal Cells

Jia Ng, Kim Hynes, Gregory White, Kisha Nandini Sivanathan, Kate Vandyke, Peter Mark Bartold, Stan Gronthos

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

MSC-like populations derived from induced pluripotent stem cells (iPSC-MSC) serve as an alternative stem cell source due to their high proliferative capacity. In this study, we assessed the immunomodulatory potential of iPSC-MSC generated from periodontal ligament (PDL) and gingival (GF) tissue. The iPSC-MSC lines exhibited a similar level of suppression of mitogen-stimulated peripheral blood mononuclear cells (PBMNC) proliferation compared to their respective parental fibroblast populations in vitro. Moreover, iPSC-MSC demonstrated the ability to suppress T-cells effector cells, Th1/Th2/Th17 populations, and increase levels of Treg cells. In order to investigate the mechanisms involved, expression of common MSC-derived soluble factors known to supress lymphocyte proliferation were assessed in iPSC-MSC cultured with PBMNC with direct cell–cell contact or separated in transwells. Real-time PCR analysis of factors known to be involved in MSC mediated immune regulation, found a general trend of elevated IDO1 and IL6 transcript levels in iPSC-MSC lines and their respective primary cells co-cultured with activated PBMNC, with a wide range of gene expression levels between the different mesenchymal cell types. The results suggest that different iPSC-MSC may be useful as a potential alternative source of cells for future clinical use in therapeutic applications because of their potent immunosuppressive properties. J. Cell. Biochem. 117: 2844–2853, 2016.

Original languageEnglish
Pages (from-to)2844-2853
Number of pages10
JournalJournal of Cellular Biochemistry
DOIs
Publication statusPublished or Issued - 1 Dec 2016

Keywords

  • GINGIVAL FIBROBLASTS
  • MESENCHYMAL STEM CELL
  • PERIODONTAL LIGAMENT CELL
  • T-CELLS
  • iPSC

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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