Imaging blood vessels and lymphatic vessels in the zebrafish

H. M. Jung, S. Isogai, Makoto Kamei, D. Castranova, A. V. Gore, B. M. Weinstein

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Citations (Scopus)

Abstract

Blood vessels supply tissues and organs with oxygen, nutrients, cellular, and humoral factors, while lymphatic vessels regulate tissue fluid homeostasis, immune trafficking, and dietary fat absorption. Understanding the mechanisms of vascular morphogenesis has become a subject of intense clinical interest because of the close association of both types of vessels with pathogenesis of a broad spectrum of human diseases. The zebrafish provides a powerful animal model to study vascular morphogenesis because of their small, accessible, and transparent embryos. These unique features of zebrafish embryos permit sophisticated high-resolution live imaging of even deeply localized vessels during embryonic development and even in adult tissues. In this chapter, we summarize various methods for blood and lymphatic vessel imaging in zebrafish, including nonvital resin injection-based or dye injection-based vessel visualization, and alkaline phosphatase staining. We also provide protocols for vital imaging of vessels using microangiography or transgenic fluorescent reporter zebrafish lines.

Original languageEnglish
Title of host publicationThe Zebrafish Cellular and Developmental Biology, Part A Cellular Biology, 2016
EditorsLeonard I. Zon, H. William Detrich, Monte Westerfield
PublisherAcademic Press Inc.
Pages69-103
Number of pages35
ISBN (Print)9780128034750
DOIs
Publication statusPublished - 1 Jan 2016

Publication series

NameMethods in Cell Biology
Volume133
ISSN (Print)0091-679X

Keywords

  • Blood
  • Endothelial
  • Imaging
  • Lymphatic
  • Microangiography
  • Microscopy
  • Morphogenesis
  • Transgenic
  • Vessel
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology

Cite this

Jung, H. M., Isogai, S., Kamei, M., Castranova, D., Gore, A. V., & Weinstein, B. M. (2016). Imaging blood vessels and lymphatic vessels in the zebrafish. In L. I. Zon, H. W. Detrich, & M. Westerfield (Eds.), The Zebrafish Cellular and Developmental Biology, Part A Cellular Biology, 2016 (pp. 69-103). (Methods in Cell Biology; Vol. 133). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2016.03.023