IFNL3 mediates interaction between innate immune cells: Implications for hepatitis C virus pathogenesis

Kate S. OConnor, Golo Ahlenstiel, Vijay Suppiah, Stephen Schibeci, Adrian Ong, Reynold Leung, David Van Der Poorten, Mark W. Douglas, Martin D. Weltman, Graeme J. Stewart, Christopher Liddle, Jacob George, David R. Booth

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Common IFN lambda 3 (IFNL3) variants have been demonstrated to affect spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. The functional basis of these genetic variants has yet to be determined. Data examining the effect of IFNL3, specifically, in innate immune cells is lacking. Here, we determined the expression of IFNL3 and its receptor IFNLR1 in blood immune cell subsets and in HCV-infected livers. Next we assessed their sensitivity to IFNL3. All participants were genotyped for the IFNL3 SNPs rs8099917 and rs12979860. Importantly, unstimulated blood immune cells express significantly higher levels of IFNL3 than HCV liver biopsies. Plasmacytoid dendritic cells (pDCs) are the predominant producers of IFNLR1, especially in response to IFN-α. PBMCs, monocytes and pDCs all respond to IFNL3 based on MxA up-regulation. No differences in IFNL3 expression levels between rs8099917 or rs12979860 genotypes were detected. This is the first study to show peripheral blood pDCs to be the main producers of IFNL3, especially compared with HCV-infected livers. This makes innate immune cells the key players in determining the functional significance of INFL3 polymorphisms in patients with HCV.

Original languageEnglish
Pages (from-to)598-605
Number of pages8
JournalInnate Immunity
Volume20
Issue number6
DOIs
Publication statusPublished - 1 Jan 2014
Externally publishedYes

Keywords

  • Hepatitis C
  • IFN
  • IFNL3
  • Innate immunity

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

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