Identification of prostate cancer-associated microRNAs in circulation using a mouse model of disease

Luke A. Selth, Scott L. Townley, Joanna L. Gillis, Wayne D. Tilley, Lisa M. Butler

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) derived from the cell-free fractions of blood are emerging as useful noninvasive markers of cancer. However, many tumors display significant molecular heterogeneity, which is likely to be reflected in the circulating miRNA fingerprints associated with that pathology. One strategy to minimize such heterogeneity is to employ genetically engineered mouse models of human cancer. Here, we describe a method to profile miRNAs in the serum of a mouse model of prostate cancer, TRansgenic Adenocarcinoma of Mouse Prostate (TRAMP), and discuss practical considerations for translating these potential biomarkers into a clinical setting.

Original languageEnglish
Title of host publicationCirculating MicroRNAs: Methods and Protocols
PublisherHumana Press Inc.
Pages235-246
Number of pages12
Volume1024
ISBN (Print)9781627034524
DOIs
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1024
ISSN (Print)10643745

Keywords

  • Circulating
  • microRNA
  • Mouse model
  • Plasma
  • Prostate cancer
  • Serum
  • TRAMP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Selth, L. A., Townley, S. L., Gillis, J. L., Tilley, W. D., & Butler, L. M. (2013). Identification of prostate cancer-associated microRNAs in circulation using a mouse model of disease. In Circulating MicroRNAs: Methods and Protocols (Vol. 1024, pp. 235-246). (Methods in Molecular Biology; Vol. 1024). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-453-1_19