Identification of homeobox genes expressed in human haemopoietic progenitor cells

Paul Moretti, Paul Simmons, Paul Thomas, David Haylock, Peter Rathjen, Mathew Vadas, Richard D'Andrea

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Homeodomain (HD)-containing proteins have been shown to regulate cellular commitment and differentiation in fungal, invertebrate and vertebrate systems. Bone marrow cells synthesizing the CD34 antigen are a complex mix of early, stem and progenitor cells at various stages of commitment to the many haemopoietic lineages. Here, we report the cloning and sequencing of 31 homeobox (HB) sequences, identified using degenerate oligodeoxyribonucleotide primers, in a polymerase chain reaction with cDNA derived from a purified CD34+ population of human haemopoietic cells. Of these sequences, 16 correspond to previously identified genes, and 13 are located within the HOXA, B and C clusters. Ten of the clones most likely represent human homologues of genes identified previously in other species. Five of the clones reported here represent novel HD sequences. The identification of five new genes using a subclass-specific 5′ primer, designed from the engrailed and Xanfl sequences, suggests that there still remain several uncharacterised HB genes in the human genome. Haemopoietic cells purified on the basis of CD34 antigen synthesis are a rich source of regulatory genes consistent with their ability to differentiate into diverse haemopoietic cell types.

Original languageEnglish
Pages (from-to)213-219
Number of pages7
JournalGene
Volume144
Issue number2
DOIs
Publication statusPublished - 8 Jul 1994

Keywords

  • CD34
  • Homeodomain
  • PCR
  • differentiation
  • haemopoiesis
  • stem cell

ASJC Scopus subject areas

  • Genetics

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