Hyaluronic acid-based nanogels improve in vivo compatibility of the anti-biofilm peptide DJK-5

Sylvia N. Kłodzińska, Daniel Pletzer, Negin Rahanjam, Thomas Rades, Robert Hancock, Hanne M. Nielsen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Anti-biofilm peptides are a subset of antimicrobial peptides and represent promising broad-spectrum agents for the treatment of bacterial biofilms, though some display host toxicity in vivo. Here we evaluated nanogels composed of modified hyaluronic acid for the encapsulation of the anti-biofilm peptide DJK-5 in vivo. Nanogels of 174 to 194 nm encapsulating 33–60% of peptide were created. Efficacy and toxicity of the nanogels were tested in vivo employing a murine abscess model of a Pseudomonas aeruginosa LESB58 high bacterial density infection. The dose of DJK-5 that could be administered intravenously to mice without inducing toxicity was more than doubled after encapsulation in nanogels. Upon subcutaneous administration, the toxicity of the DJK-5 in nanogels was decreased four-fold compared to non-formulated peptide, without compromising the anti-abscess effect of DJK-5. These findings support the use of nanogels to increase the safety of antimicrobial and anti-biofilm peptides after intravenous and subcutaneous administration.

Original languageEnglish
Article number102022
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume20
DOIs
Publication statusPublished - 1 Aug 2019
Externally publishedYes

Keywords

  • Biofilm
  • Cationic peptide
  • Drug delivery
  • Nanogel
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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