Human α-L-fucosidase: Complete coding sequence form cDNA clones

Teresa Occhiodoro, Kerri R. Beckmann, C. Phillip Morris, John J. Hopwood

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The human lysosomal storage disorder fucosidosis results from the deficiency of α-L-fucosidase, a lysosomal enzyme essential for the catabolism of oligosaccharides containing α-L-fucosides. cDNA clones coding for human α-L-fucosidase have been isolated from λgt10 and λgtll cDNA libraries derived from human liver, placenta and colon. Compilation of cDNA sequences results in a nucleotide sequence of 2053 base pairs encoding α-L-fucosidase. The sequence contains an open reading frame of 461 amino acids beginning with the first in-frame methionine and includes 439 amino acids which comprise the mature protein in addition to a hydrophobic signal peptide sequence of 22 amino acids.

Original languageEnglish
Pages (from-to)439-445
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number1
DOIs
Publication statusPublished or Issued - 16 Oct 1989

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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