HLA-DR, DQ and anti-GAD antibodies in first degree relatives of type I diabetes mellitus

M. Serrano-Ríos, M. D. Gutierrez-López, F. Pérez-Bravo, M. T. Martínez, J. Antona, M. Rowley, I. Mackay, Paul Zimmet

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12 Citations (Scopus)


The differential antibody response to glutamic acid decarboxylase (anti-GAD) and to islet cell cytoplasm (ICA) according to HLA-DR and DQ genotypes were examined in 28 Spanish patients with Type I diabetes mellitus (11.1 ± 10.4 year diabetes duration) and their 41 first degree non-diabetic relatives. Anti-GAD was detected by radioimmunoprecipitation and ICA by indirect immunofluorescence and HLA-DR/DQ alleles were assigned by PCR and sequence specific oligonucleotide probes. The frequency in patients of positivity for ICA was 7.1% and of anti-GAD+ 64.3% and in relatives, the frequency of ICA+ was 4.9%, and anti-GAD+ 9.8%. Concurrent positivity for ICA and anti-GAD existed in only one patient, and in none of the relatives. We confirm for a Spanish population the high frequency of risk genotypes for Type I, involving DR3, DR4 and DQB1*0302 (DQ8), which were present in 26 of 28 (93%) patients and 32 of 41 (78%) relatives. The most frequent genotypes were DR3/DQB1*0201/DQA1*0501-DR4/DQB1*0302/DQA1*0301 (9 patients, 32%; 6 relatives, 15%), DR3/DQB1*0201/DQA1*0501-DR3/DQB1*0201/DQA1*0501 (5 patients, 18%; 7 relatives, 17%) and DR3/DQB1*0201/DQA1*0501DR1/DQB1*0501/DQA1*0l01 (5 patients, 18%; 1 relative, 2%). Positivity for anti-GAD or for ICA did not correlate with gender, or age at onset or duration of DM. The distribution of high risk HLA genotypes were similar regardless of the anti-GAD or anti-ICA status either in patients or in their relatives.

Original languageEnglish
JournalDiabetes Research and Clinical Practice
Issue numberSUPPL.
Publication statusPublished - 1 Jan 1996


  • Spanish families
  • glutamic acid decarboxylase
  • islet cell cytoplasm
  • type I (insulin-dependent) diabetes mellitus

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Serrano-Ríos, M., Gutierrez-López, M. D., Pérez-Bravo, F., Martínez, M. T., Antona, J., Rowley, M., ... Zimmet, P. (1996). HLA-DR, DQ and anti-GAD antibodies in first degree relatives of type I diabetes mellitus. Diabetes Research and Clinical Practice, 34(SUPPL.). https://doi.org/10.1016/S0168-8227(96)01316-2