HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles

Lachlan R. Gray, Stuart G. Turville, Tina L. HItchen, Wan Jung Cheng, Anne M. Ellett, Hamid Salimi, Michael J. Roche, Steve L. Wesselingh, Paul R. Gorry, Melissa J. Churchill

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Astrocytes are extensively infected with HIV-1 in vivo and play a significant role in the development of HIV-1-associated neurocognitive disorders. Despite their extensive infection, little is known about how astrocytes become infected, since they lack cell surface CD4 expression. In the present study, we investigated the fate of HIV-1 upon infection of astrocytes. Astrocytes were found to bind and harbor virus followed by biphasic decay, with HIV-1 detectable out to 72 hours. HIV-1 was observed to associate with CD81-lined vesicle structures. shRNA silencing of CD81 resulted in less cell-associated virus but no loss of co-localization between HIV-1 and CD81. Astrocytes supported trans-infection of HIV-1 to T-cells without de novo virus production, and the virus-containing compartment required 37uC to form, and was trypsin-resistant. The CD81 compartment observed herein, has been shown in other cell types to be a relatively protective compartment. Within astrocytes, this compartment may be actively involved in virus entry and/or spread. The ability of astrocytes to transfer virus, without de novo viral synthesis suggests they are capable of sequestering and protecting virus and thus, they could potentially facilitate viral dissemination in the CNS

LanguageEnglish
Article numbere90620
JournalPLoS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 28 Feb 2014

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Gray, L. R., Turville, S. G., HItchen, T. L., Cheng, W. J., Ellett, A. M., Salimi, H., ... Churchill, M. J. (2014). HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles. PLoS ONE, 9(2), [e90620]. https://doi.org/10.1371/journal.pone.0090620
Gray, Lachlan R. ; Turville, Stuart G. ; HItchen, Tina L. ; Cheng, Wan Jung ; Ellett, Anne M. ; Salimi, Hamid ; Roche, Michael J. ; Wesselingh, Steve L. ; Gorry, Paul R. ; Churchill, Melissa J. / HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles. In: PLoS ONE. 2014 ; Vol. 9, No. 2.
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Gray, LR, Turville, SG, HItchen, TL, Cheng, WJ, Ellett, AM, Salimi, H, Roche, MJ, Wesselingh, SL, Gorry, PR & Churchill, MJ 2014, 'HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles', PLoS ONE, vol. 9, no. 2, e90620. https://doi.org/10.1371/journal.pone.0090620

HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles. / Gray, Lachlan R.; Turville, Stuart G.; HItchen, Tina L.; Cheng, Wan Jung; Ellett, Anne M.; Salimi, Hamid; Roche, Michael J.; Wesselingh, Steve L.; Gorry, Paul R.; Churchill, Melissa J.

In: PLoS ONE, Vol. 9, No. 2, e90620, 28.02.2014.

Research output: Contribution to journalArticle

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Gray LR, Turville SG, HItchen TL, Cheng WJ, Ellett AM, Salimi H et al. HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles. PLoS ONE. 2014 Feb 28;9(2). e90620. https://doi.org/10.1371/journal.pone.0090620