Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution

Enkhjargal Tsend-Ayush, Natasha Dodge, Julia Mohr, Aaron Casey, Heinz Himmelbauer, Colin L. Kremitzki, Kyriena Schatzkamer, Tina Graves, Wesley C. Warren, Frank Griützner

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In mammals, chromosomes occupy defined positions in sperm, whereas previous work in chicken showed random chromosome distribution. Monotremes (platypus and echidnas) are the most basal group of living mammals. They have elongated sperm like chicken and a complex sex chromosome system with homology to chicken sex chromosomes. We used platypus and chicken genomic clones to investigate genome organization in sperm. In chicken sperm, about half of the chromosomes investigated are organized non-randomly, whereas in platypus chromosome organization in sperm is almost entirely non-random. The use of genomic clones allowed us to determine chromosome orientation and chromatin compaction in sperm. We found that in both species chromosomes maintain orientation of chromosomes in sperm independent of random or non-random positioning along the sperm nucleus. The distance of loci correlated with the total length of sperm nuclei, suggesting that chromatin extension depends on sperm elongation. In platypus, most sex chromosomes cluster in the posterior region of the sperm nucleus, presumably the result of postmeiotic association of sex chromosomes. Chicken and platypus autosomes sharing homology with the human X chromosome located centrally in both species suggesting that this is the ancestral position. This suggests that in some therian mammals a more anterior position of the X chromosome has evolved independently.

LanguageEnglish
Pages53-69
Number of pages17
JournalChromosoma
Volume118
Issue number1
DOIs
Publication statusPublished - 1 Jan 2009

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Tsend-Ayush, Enkhjargal ; Dodge, Natasha ; Mohr, Julia ; Casey, Aaron ; Himmelbauer, Heinz ; Kremitzki, Colin L. ; Schatzkamer, Kyriena ; Graves, Tina ; Warren, Wesley C. ; Griützner, Frank. / Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution. In: Chromosoma. 2009 ; Vol. 118, No. 1. pp. 53-69.
@article{97ca81957c0b47bba157a6905afeaa03,
title = "Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution",
abstract = "In mammals, chromosomes occupy defined positions in sperm, whereas previous work in chicken showed random chromosome distribution. Monotremes (platypus and echidnas) are the most basal group of living mammals. They have elongated sperm like chicken and a complex sex chromosome system with homology to chicken sex chromosomes. We used platypus and chicken genomic clones to investigate genome organization in sperm. In chicken sperm, about half of the chromosomes investigated are organized non-randomly, whereas in platypus chromosome organization in sperm is almost entirely non-random. The use of genomic clones allowed us to determine chromosome orientation and chromatin compaction in sperm. We found that in both species chromosomes maintain orientation of chromosomes in sperm independent of random or non-random positioning along the sperm nucleus. The distance of loci correlated with the total length of sperm nuclei, suggesting that chromatin extension depends on sperm elongation. In platypus, most sex chromosomes cluster in the posterior region of the sperm nucleus, presumably the result of postmeiotic association of sex chromosomes. Chicken and platypus autosomes sharing homology with the human X chromosome located centrally in both species suggesting that this is the ancestral position. This suggests that in some therian mammals a more anterior position of the X chromosome has evolved independently.",
author = "Enkhjargal Tsend-Ayush and Natasha Dodge and Julia Mohr and Aaron Casey and Heinz Himmelbauer and Kremitzki, {Colin L.} and Kyriena Schatzkamer and Tina Graves and Warren, {Wesley C.} and Frank Gri{\"u}tzner",
year = "2009",
month = "1",
day = "1",
doi = "10.1007/s00412-008-0177-1",
language = "English",
volume = "118",
pages = "53--69",
journal = "Chromosoma",
issn = "0009-5915",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1",

}

Tsend-Ayush, E, Dodge, N, Mohr, J, Casey, A, Himmelbauer, H, Kremitzki, CL, Schatzkamer, K, Graves, T, Warren, WC & Griützner, F 2009, 'Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution', Chromosoma, vol. 118, no. 1, pp. 53-69. https://doi.org/10.1007/s00412-008-0177-1

Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution. / Tsend-Ayush, Enkhjargal; Dodge, Natasha; Mohr, Julia; Casey, Aaron; Himmelbauer, Heinz; Kremitzki, Colin L.; Schatzkamer, Kyriena; Graves, Tina; Warren, Wesley C.; Griützner, Frank.

In: Chromosoma, Vol. 118, No. 1, 01.01.2009, p. 53-69.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Higher-order genome organization in platypus and chicken sperm and repositioning of sex chromosomes during mammalian evolution

AU - Tsend-Ayush, Enkhjargal

AU - Dodge, Natasha

AU - Mohr, Julia

AU - Casey, Aaron

AU - Himmelbauer, Heinz

AU - Kremitzki, Colin L.

AU - Schatzkamer, Kyriena

AU - Graves, Tina

AU - Warren, Wesley C.

AU - Griützner, Frank

PY - 2009/1/1

Y1 - 2009/1/1

N2 - In mammals, chromosomes occupy defined positions in sperm, whereas previous work in chicken showed random chromosome distribution. Monotremes (platypus and echidnas) are the most basal group of living mammals. They have elongated sperm like chicken and a complex sex chromosome system with homology to chicken sex chromosomes. We used platypus and chicken genomic clones to investigate genome organization in sperm. In chicken sperm, about half of the chromosomes investigated are organized non-randomly, whereas in platypus chromosome organization in sperm is almost entirely non-random. The use of genomic clones allowed us to determine chromosome orientation and chromatin compaction in sperm. We found that in both species chromosomes maintain orientation of chromosomes in sperm independent of random or non-random positioning along the sperm nucleus. The distance of loci correlated with the total length of sperm nuclei, suggesting that chromatin extension depends on sperm elongation. In platypus, most sex chromosomes cluster in the posterior region of the sperm nucleus, presumably the result of postmeiotic association of sex chromosomes. Chicken and platypus autosomes sharing homology with the human X chromosome located centrally in both species suggesting that this is the ancestral position. This suggests that in some therian mammals a more anterior position of the X chromosome has evolved independently.

AB - In mammals, chromosomes occupy defined positions in sperm, whereas previous work in chicken showed random chromosome distribution. Monotremes (platypus and echidnas) are the most basal group of living mammals. They have elongated sperm like chicken and a complex sex chromosome system with homology to chicken sex chromosomes. We used platypus and chicken genomic clones to investigate genome organization in sperm. In chicken sperm, about half of the chromosomes investigated are organized non-randomly, whereas in platypus chromosome organization in sperm is almost entirely non-random. The use of genomic clones allowed us to determine chromosome orientation and chromatin compaction in sperm. We found that in both species chromosomes maintain orientation of chromosomes in sperm independent of random or non-random positioning along the sperm nucleus. The distance of loci correlated with the total length of sperm nuclei, suggesting that chromatin extension depends on sperm elongation. In platypus, most sex chromosomes cluster in the posterior region of the sperm nucleus, presumably the result of postmeiotic association of sex chromosomes. Chicken and platypus autosomes sharing homology with the human X chromosome located centrally in both species suggesting that this is the ancestral position. This suggests that in some therian mammals a more anterior position of the X chromosome has evolved independently.

UR - http://www.scopus.com/inward/record.url?scp=58349104767&partnerID=8YFLogxK

U2 - 10.1007/s00412-008-0177-1

DO - 10.1007/s00412-008-0177-1

M3 - Article

VL - 118

SP - 53

EP - 69

JO - Chromosoma

T2 - Chromosoma

JF - Chromosoma

SN - 0009-5915

IS - 1

ER -