High-Energy Diet and Shorter Light Exposure Drives Markers of Adipocyte Dysfunction in Visceral and Subcutaneous Adipose Depots of Psammomys obesus

Joanne T M Tan, Victoria A Nankivell, Carmel Bilu, Tomer Shemesh, Stephen J Nicholls, Paul Zimmet, Noga Kronfeld-Schor, Alex Brown, Christina A Bursill

Research output: Contribution to journalArticle

Abstract

Dysfunctional adipose tissue phenotype underpins type 2 diabetes mellitus (T2DM) development. The disruption of circadian rhythms contributes to T2DM development. We investigated the effects of high-energy diet and photoperiod length on visceral and subcutaneous adipose tissue phenotype. Psammomys obesus sand rats exposed to neutral (12 light:12 dark) or short (5 light:19 dark) photoperiod were fed a low- (LE) or high- (HE) energy diet. The HE diet and/or short photoperiod reduced subcutaneous expression of adipocyte differentiation/function markers C/ebpα, Pparδ, Pparγ and Adipoq. Visceral Pparα levels were elevated in the 5:19HE group; however, the HE diet and/or short photoperiod decreased visceral Pparγ and Adipoq expression. 5:19HE animals had elevated Ucp1 yet lower Pgc-1α levels. The HE diet increased visceral Tgf-β1, Ccl2 and Cd68 levels, suggestive of a pro-inflammatory state. Daily visceral rhythms of these genes were affected by a short photoperiod and/or HE diet. The 12:12HE, 5:19LE or 5:19HE animals had a higher proportion of larger adipocytes, indicating increased adipocyte hypertrophy. Collectively, the HE diet and/or shorter light exposure drives a dysfunctional adipose tissue phenotype. Daily rhythms are affected by a short photoperiod and HE diet in a site-specific manner. These findings provide mechanistic insight on the influence of disrupted circadian rhythms and HE diet on adipose tissue phenotype.

LanguageEnglish
JournalInternational Journal of Molecular Sciences
Volume20
Issue number24
DOIs
Publication statusPublished - 13 Dec 2019
Externally publishedYes

Keywords

  • Adipocyte Dysfunction
  • Visceral and Subcutaneous Adipose Depots
  • Psammomys obesus
  • type 2 diabetes mellitus (T2DM)
  • photoperiod
  • C/ebpα
  • Pparδ
  • Pparγ
  • Adipoq
  • adipocyte differentiation
  • adipocyte hypertrophy
  • browning
  • circadian rhythms
  • inflammation

Cite this

@article{43e2172d27364e119edeb46679a7d5c8,
title = "High-Energy Diet and Shorter Light Exposure Drives Markers of Adipocyte Dysfunction in Visceral and Subcutaneous Adipose Depots of Psammomys obesus",
abstract = "Dysfunctional adipose tissue phenotype underpins type 2 diabetes mellitus (T2DM) development. The disruption of circadian rhythms contributes to T2DM development. We investigated the effects of high-energy diet and photoperiod length on visceral and subcutaneous adipose tissue phenotype. Psammomys obesus sand rats exposed to neutral (12 light:12 dark) or short (5 light:19 dark) photoperiod were fed a low- (LE) or high- (HE) energy diet. The HE diet and/or short photoperiod reduced subcutaneous expression of adipocyte differentiation/function markers C/ebpα, Pparδ, Pparγ and Adipoq. Visceral Pparα levels were elevated in the 5:19HE group; however, the HE diet and/or short photoperiod decreased visceral Pparγ and Adipoq expression. 5:19HE animals had elevated Ucp1 yet lower Pgc-1α levels. The HE diet increased visceral Tgf-β1, Ccl2 and Cd68 levels, suggestive of a pro-inflammatory state. Daily visceral rhythms of these genes were affected by a short photoperiod and/or HE diet. The 12:12HE, 5:19LE or 5:19HE animals had a higher proportion of larger adipocytes, indicating increased adipocyte hypertrophy. Collectively, the HE diet and/or shorter light exposure drives a dysfunctional adipose tissue phenotype. Daily rhythms are affected by a short photoperiod and HE diet in a site-specific manner. These findings provide mechanistic insight on the influence of disrupted circadian rhythms and HE diet on adipose tissue phenotype.",
keywords = "Adipocyte Dysfunction, Visceral and Subcutaneous Adipose Depots, Psammomys obesus, type 2 diabetes mellitus (T2DM), photoperiod, C/ebpα, Pparδ, Pparγ, Adipoq, adipocyte differentiation, adipocyte hypertrophy, browning, circadian rhythms, inflammation",
author = "Tan, {Joanne T M} and Nankivell, {Victoria A} and Carmel Bilu and Tomer Shemesh and Nicholls, {Stephen J} and Paul Zimmet and Noga Kronfeld-Schor and Alex Brown and Bursill, {Christina A}",
year = "2019",
month = "12",
day = "13",
doi = "10.3390/ijms20246291",
language = "English",
volume = "20",
journal = "International Journal of Molecular Sciences",
issn = "1661-6596",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
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High-Energy Diet and Shorter Light Exposure Drives Markers of Adipocyte Dysfunction in Visceral and Subcutaneous Adipose Depots of Psammomys obesus. / Tan, Joanne T M; Nankivell, Victoria A; Bilu, Carmel; Shemesh, Tomer; Nicholls, Stephen J; Zimmet, Paul; Kronfeld-Schor, Noga; Brown, Alex; Bursill, Christina A.

In: International Journal of Molecular Sciences, Vol. 20, No. 24, 13.12.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - High-Energy Diet and Shorter Light Exposure Drives Markers of Adipocyte Dysfunction in Visceral and Subcutaneous Adipose Depots of Psammomys obesus

AU - Tan, Joanne T M

AU - Nankivell, Victoria A

AU - Bilu, Carmel

AU - Shemesh, Tomer

AU - Nicholls, Stephen J

AU - Zimmet, Paul

AU - Kronfeld-Schor, Noga

AU - Brown, Alex

AU - Bursill, Christina A

PY - 2019/12/13

Y1 - 2019/12/13

N2 - Dysfunctional adipose tissue phenotype underpins type 2 diabetes mellitus (T2DM) development. The disruption of circadian rhythms contributes to T2DM development. We investigated the effects of high-energy diet and photoperiod length on visceral and subcutaneous adipose tissue phenotype. Psammomys obesus sand rats exposed to neutral (12 light:12 dark) or short (5 light:19 dark) photoperiod were fed a low- (LE) or high- (HE) energy diet. The HE diet and/or short photoperiod reduced subcutaneous expression of adipocyte differentiation/function markers C/ebpα, Pparδ, Pparγ and Adipoq. Visceral Pparα levels were elevated in the 5:19HE group; however, the HE diet and/or short photoperiod decreased visceral Pparγ and Adipoq expression. 5:19HE animals had elevated Ucp1 yet lower Pgc-1α levels. The HE diet increased visceral Tgf-β1, Ccl2 and Cd68 levels, suggestive of a pro-inflammatory state. Daily visceral rhythms of these genes were affected by a short photoperiod and/or HE diet. The 12:12HE, 5:19LE or 5:19HE animals had a higher proportion of larger adipocytes, indicating increased adipocyte hypertrophy. Collectively, the HE diet and/or shorter light exposure drives a dysfunctional adipose tissue phenotype. Daily rhythms are affected by a short photoperiod and HE diet in a site-specific manner. These findings provide mechanistic insight on the influence of disrupted circadian rhythms and HE diet on adipose tissue phenotype.

AB - Dysfunctional adipose tissue phenotype underpins type 2 diabetes mellitus (T2DM) development. The disruption of circadian rhythms contributes to T2DM development. We investigated the effects of high-energy diet and photoperiod length on visceral and subcutaneous adipose tissue phenotype. Psammomys obesus sand rats exposed to neutral (12 light:12 dark) or short (5 light:19 dark) photoperiod were fed a low- (LE) or high- (HE) energy diet. The HE diet and/or short photoperiod reduced subcutaneous expression of adipocyte differentiation/function markers C/ebpα, Pparδ, Pparγ and Adipoq. Visceral Pparα levels were elevated in the 5:19HE group; however, the HE diet and/or short photoperiod decreased visceral Pparγ and Adipoq expression. 5:19HE animals had elevated Ucp1 yet lower Pgc-1α levels. The HE diet increased visceral Tgf-β1, Ccl2 and Cd68 levels, suggestive of a pro-inflammatory state. Daily visceral rhythms of these genes were affected by a short photoperiod and/or HE diet. The 12:12HE, 5:19LE or 5:19HE animals had a higher proportion of larger adipocytes, indicating increased adipocyte hypertrophy. Collectively, the HE diet and/or shorter light exposure drives a dysfunctional adipose tissue phenotype. Daily rhythms are affected by a short photoperiod and HE diet in a site-specific manner. These findings provide mechanistic insight on the influence of disrupted circadian rhythms and HE diet on adipose tissue phenotype.

KW - Adipocyte Dysfunction

KW - Visceral and Subcutaneous Adipose Depots

KW - Psammomys obesus

KW - type 2 diabetes mellitus (T2DM)

KW - photoperiod

KW - C/ebpα

KW - Pparδ

KW - Pparγ

KW - Adipoq

KW - adipocyte differentiation

KW - adipocyte hypertrophy

KW - browning

KW - circadian rhythms

KW - inflammation

U2 - 10.3390/ijms20246291

DO - 10.3390/ijms20246291

M3 - Article

VL - 20

JO - International Journal of Molecular Sciences

T2 - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 24

ER -