Abstract
Haemoglobin expression is not restricted to erythroid cells. We investigated the gene expression of the haemoglobin subunits haemoglobin, alpha adult chain 1 (Hba-a1) and haemoglobin, beta (Hbb), 2,3-bisphosphoglycerate mutase (Bpgm) and the oxygen-regulated genes BCL2/adenovirus E1B interacting protein 3 (Bnip3), solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1) and N-myc downstream regulated gene 1 (Ndrg1) in the murine preimplantation embryo, comparing in vivo to in vitro gene expression. Relatively high levels of Hba-a1 and Hbb were expressed in vivo from the 2-cell to blastocyst stage; in contrast, little or no expression occurred in vitro. We hypothesised that the presence of haemoglobin in vivo creates a low oxygen environment to induce oxygen-regulated gene expression, supported by high expression of Slc2a1 and Ndrg1 in in vivo relative to in vitro embryos. In addition, analysis of an in vitro-derived human embryo gene expression public dataset revealed low expression of haemoglobin subunit alpha (HBA) and HBB, and high expression of BPGM. To explore whether there was a developmental stage-specific effect of haemoglobin, we added exogenous haemoglobin either up to the 4-cell stage or throughout development to the blastocyst stage, but observed no difference in blastocyst rate or the inner cell mass to trophectoderm cell ratio. We conclude that haemoglobin in the in vivo preimplantation embryo raises an interesting premise of potential mechanisms for oxygen regulation, which may influence oxygen-regulated gene expression.
| Language | English |
|---|---|
| Pages | 724-734 |
| Number of pages | 11 |
| Journal | Reproduction, Fertility and Development |
| Volume | 31 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Keywords
- IVF
- gene regulation
ASJC Scopus subject areas
- Biotechnology
- Reproductive Medicine
- Animal Science and Zoology
- Molecular Biology
- Genetics
- Endocrinology
- Developmental Biology
Cite this
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Haemoglobin expression in in vivo murine preimplantation embryos suggests a role in oxygen-regulated gene expression. / Lim, M.; Brown, H. M.; Kind, K. L.; Breen, J.; Anastasi, M. R.; Ritter, L. J.; Tregoweth, E. K.; Dinh, D. T.; Thompson, J. G.; Dunning, K. R.
In: Reproduction, Fertility and Development, Vol. 31, No. 4, 01.01.2019, p. 724-734.Research output: Contribution to journal › Article
TY - JOUR
T1 - Haemoglobin expression in in vivo murine preimplantation embryos suggests a role in oxygen-regulated gene expression
AU - Lim, M.
AU - Brown, H. M.
AU - Kind, K. L.
AU - Breen, J.
AU - Anastasi, M. R.
AU - Ritter, L. J.
AU - Tregoweth, E. K.
AU - Dinh, D. T.
AU - Thompson, J. G.
AU - Dunning, K. R.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Haemoglobin expression is not restricted to erythroid cells. We investigated the gene expression of the haemoglobin subunits haemoglobin, alpha adult chain 1 (Hba-a1) and haemoglobin, beta (Hbb), 2,3-bisphosphoglycerate mutase (Bpgm) and the oxygen-regulated genes BCL2/adenovirus E1B interacting protein 3 (Bnip3), solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1) and N-myc downstream regulated gene 1 (Ndrg1) in the murine preimplantation embryo, comparing in vivo to in vitro gene expression. Relatively high levels of Hba-a1 and Hbb were expressed in vivo from the 2-cell to blastocyst stage; in contrast, little or no expression occurred in vitro. We hypothesised that the presence of haemoglobin in vivo creates a low oxygen environment to induce oxygen-regulated gene expression, supported by high expression of Slc2a1 and Ndrg1 in in vivo relative to in vitro embryos. In addition, analysis of an in vitro-derived human embryo gene expression public dataset revealed low expression of haemoglobin subunit alpha (HBA) and HBB, and high expression of BPGM. To explore whether there was a developmental stage-specific effect of haemoglobin, we added exogenous haemoglobin either up to the 4-cell stage or throughout development to the blastocyst stage, but observed no difference in blastocyst rate or the inner cell mass to trophectoderm cell ratio. We conclude that haemoglobin in the in vivo preimplantation embryo raises an interesting premise of potential mechanisms for oxygen regulation, which may influence oxygen-regulated gene expression.
AB - Haemoglobin expression is not restricted to erythroid cells. We investigated the gene expression of the haemoglobin subunits haemoglobin, alpha adult chain 1 (Hba-a1) and haemoglobin, beta (Hbb), 2,3-bisphosphoglycerate mutase (Bpgm) and the oxygen-regulated genes BCL2/adenovirus E1B interacting protein 3 (Bnip3), solute carrier family 2 (facilitated glucose transporter), member 1 (Slc2a1) and N-myc downstream regulated gene 1 (Ndrg1) in the murine preimplantation embryo, comparing in vivo to in vitro gene expression. Relatively high levels of Hba-a1 and Hbb were expressed in vivo from the 2-cell to blastocyst stage; in contrast, little or no expression occurred in vitro. We hypothesised that the presence of haemoglobin in vivo creates a low oxygen environment to induce oxygen-regulated gene expression, supported by high expression of Slc2a1 and Ndrg1 in in vivo relative to in vitro embryos. In addition, analysis of an in vitro-derived human embryo gene expression public dataset revealed low expression of haemoglobin subunit alpha (HBA) and HBB, and high expression of BPGM. To explore whether there was a developmental stage-specific effect of haemoglobin, we added exogenous haemoglobin either up to the 4-cell stage or throughout development to the blastocyst stage, but observed no difference in blastocyst rate or the inner cell mass to trophectoderm cell ratio. We conclude that haemoglobin in the in vivo preimplantation embryo raises an interesting premise of potential mechanisms for oxygen regulation, which may influence oxygen-regulated gene expression.
KW - IVF
KW - gene regulation
UR - http://www.scopus.com/inward/record.url?scp=85057781605&partnerID=8YFLogxK
U2 - 10.1071/RD17321
DO - 10.1071/RD17321
M3 - Article
VL - 31
SP - 724
EP - 734
JO - Reproduction, Fertility and Development
T2 - Reproduction, Fertility and Development
JF - Reproduction, Fertility and Development
SN - 1031-3613
IS - 4
ER -