Gestational dietary protein is associated with sex specific decrease in blood flow, fetal heart growth and post-natal blood pressure of progeny

Juan H. Hernandez-Medrano, Katrina J. Copping, Andrew Hoare, Wendela Wapanaar, Rosalie Grivell, Tim Kuchel, Giuliana Miguel-Pacheco, I. Caroline McMillen, Raymond J. Rodgers, Viv E A Perry

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Study Overview: The incidence of adverse pregnancy outcomes is higher in pregnancies where the fetus is male. Sex specific differences in feto-placental perfusion indices identified by Doppler assessment have recently been associated with placental insufficiency and fetal growth restriction. This study aims to investigate sex specific differences in placental perfusion and to correlate these changes with fetal growth. It represents the largest comprehensive study under field conditions of uterine hemodynamics in a monotocous species, with a similar long gestation period to the human. Primiparous 14mo heifers in Australia (n=360) and UK (n=180) were either individually or group fed, respectively, diets with differing protein content (18, 14, 10 or 7% crude protein (CP)) from 60d prior to 98 days post conception (dpc). Fetuses and placentae were excised at 98dpc (n = 48). Fetal development an median uterine artery blood flow were assessed monthly from 36dpc until term using B-mode and Doppler ultrasonography. MUA blood flow to the male feto-placental unit increased in early pregnancy associated with increased fetal growth. Protein restriction before and shortly after conception (-60d up to 23dpc) increased MUA diameter and indices of velocity during late pregnancy, reduced fetal heart weight in the female fetus and increased heart rate at birth, but decreased systolic blood pressure at six months of age. Conclusion and Significance: Sex specific differences both in feto-placental Doppler perfusion indices and response of these indices to dietary perturbations were observed. Further, maternal diet affected development of fetal cardiovascular system associated with altered fetal haemodynamics in utero, with such effects having a sex bias. The results from this study provide further insight into the gender specific circulatory differences present in the fetal period and developing cardiovascular system.

LanguageEnglish
Article numbere0125694
JournalPLoS ONE
Volume10
Issue number4
DOIs
Publication statusPublished - 27 Apr 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hernandez-Medrano, Juan H. ; Copping, Katrina J. ; Hoare, Andrew ; Wapanaar, Wendela ; Grivell, Rosalie ; Kuchel, Tim ; Miguel-Pacheco, Giuliana ; McMillen, I. Caroline ; Rodgers, Raymond J. ; Perry, Viv E A. / Gestational dietary protein is associated with sex specific decrease in blood flow, fetal heart growth and post-natal blood pressure of progeny. In: PLoS ONE. 2015 ; Vol. 10, No. 4.
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abstract = "Study Overview: The incidence of adverse pregnancy outcomes is higher in pregnancies where the fetus is male. Sex specific differences in feto-placental perfusion indices identified by Doppler assessment have recently been associated with placental insufficiency and fetal growth restriction. This study aims to investigate sex specific differences in placental perfusion and to correlate these changes with fetal growth. It represents the largest comprehensive study under field conditions of uterine hemodynamics in a monotocous species, with a similar long gestation period to the human. Primiparous 14mo heifers in Australia (n=360) and UK (n=180) were either individually or group fed, respectively, diets with differing protein content (18, 14, 10 or 7{\%} crude protein (CP)) from 60d prior to 98 days post conception (dpc). Fetuses and placentae were excised at 98dpc (n = 48). Fetal development an median uterine artery blood flow were assessed monthly from 36dpc until term using B-mode and Doppler ultrasonography. MUA blood flow to the male feto-placental unit increased in early pregnancy associated with increased fetal growth. Protein restriction before and shortly after conception (-60d up to 23dpc) increased MUA diameter and indices of velocity during late pregnancy, reduced fetal heart weight in the female fetus and increased heart rate at birth, but decreased systolic blood pressure at six months of age. Conclusion and Significance: Sex specific differences both in feto-placental Doppler perfusion indices and response of these indices to dietary perturbations were observed. Further, maternal diet affected development of fetal cardiovascular system associated with altered fetal haemodynamics in utero, with such effects having a sex bias. The results from this study provide further insight into the gender specific circulatory differences present in the fetal period and developing cardiovascular system.",
author = "Hernandez-Medrano, {Juan H.} and Copping, {Katrina J.} and Andrew Hoare and Wendela Wapanaar and Rosalie Grivell and Tim Kuchel and Giuliana Miguel-Pacheco and McMillen, {I. Caroline} and Rodgers, {Raymond J.} and Perry, {Viv E A}",
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Hernandez-Medrano, JH, Copping, KJ, Hoare, A, Wapanaar, W, Grivell, R, Kuchel, T, Miguel-Pacheco, G, McMillen, IC, Rodgers, RJ & Perry, VEA 2015, 'Gestational dietary protein is associated with sex specific decrease in blood flow, fetal heart growth and post-natal blood pressure of progeny', PLoS ONE, vol. 10, no. 4, e0125694. https://doi.org/10.1371/journal.pone.0125694

Gestational dietary protein is associated with sex specific decrease in blood flow, fetal heart growth and post-natal blood pressure of progeny. / Hernandez-Medrano, Juan H.; Copping, Katrina J.; Hoare, Andrew; Wapanaar, Wendela; Grivell, Rosalie; Kuchel, Tim; Miguel-Pacheco, Giuliana; McMillen, I. Caroline; Rodgers, Raymond J.; Perry, Viv E A.

In: PLoS ONE, Vol. 10, No. 4, e0125694, 27.04.2015.

Research output: Contribution to journalArticle

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AU - Hernandez-Medrano, Juan H.

AU - Copping, Katrina J.

AU - Hoare, Andrew

AU - Wapanaar, Wendela

AU - Grivell, Rosalie

AU - Kuchel, Tim

AU - Miguel-Pacheco, Giuliana

AU - McMillen, I. Caroline

AU - Rodgers, Raymond J.

AU - Perry, Viv E A

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