Gene expression microarray analysis of early oxygen-induced retinopathy in the rat

Melinda Tea, Rhys Fogarty, Helen M. Brereton, Michael Z. Michael, Mark B. Van der Hoek, Anna Tsykin, Douglas J. Coster, Keryn A. Williams

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Different inbred strains of rat differ in their susceptibility to oxygen-induced retinopathy (OIR), an animal model of human retinopathy of prematurity. We examined gene expression in Sprague-Dawley (susceptible) and Fischer 344 (resistant) neonatal rats after 3 days exposure to cyclic hyperoxia or room air, using Affymetrix rat Genearrays. False discovery rate analysis was used to identify differentially regulated genes. Such genes were then ranked by fold change and submitted to the online database, DAVID. The Sprague-Dawley list returned the term "response to hypoxia," absent from the Fischer 344 output. Manual analysis indicated that many genes known to be upregulated by hypoxia-inducible factor-1α were down-regulated by cyclic hyperoxia. Quantitative real-time RTPCR analysis of Egln3, Bnip3, Slc16a3, and Hk2 confirmed the microarray results. We conclude that combined methodologies are required for adequate dissection of the pathophysiology of strain susceptibility to OIR in the rat.

Original languageEnglish
Pages (from-to)190-201
Number of pages12
JournalJournal of Ocular Biology, Diseases, and Informatics
Issue number4
Publication statusPublished or Issued - Dec 2009


  • Affymetrix microarray
  • Cyclic hyperoxia
  • Gene expression
  • Inbred rat
  • Oxygen-induced retinopathy

ASJC Scopus subject areas

  • Ophthalmology
  • Genetics

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