Fusion Pore Size Limits 5-HT Release From Single Enterochromaffin Cell Vesicles

Ravinarayan Raghupathi, Claire F. Jessup, Amanda Lumsden, Damien J. Keating

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Enterochromaffin cells are the major site of serotonin (5-HT) synthesis and secretion providing ∼95% of the body's total 5-HT. 5-HT can act as a neurotransmitter or hormone and has several important endocrine and paracrine roles. We have previously demonstrated that EC cells release small amounts of 5-HT per exocytosis event compared to other endocrine cells. We utilized a recently developed method to purify EC cells to demonstrate the mechanisms underlying 5-HT packaging and release. Using the fluorescent probe FFN511, we demonstrate that EC cells express VMAT and that VMAT plays a functional role in 5-HT loading into vesicles. Carbon fiber amperometry studies illustrate that the amount of 5-HT released per exocytosis event from EC cells is dependent on both VMAT and the H+-ATPase pump, as demonstrated with reserpine or bafilomycin, respectively. We also demonstrate that increasing the amount of 5-HT loaded into EC cell vesicles does not result in an increase in quantal release. As this indicates that fusion pore size may be a limiting factor involved, we compared pore diameter in EC and chromaffin cells by assessing the vesicle capture of different-sized fluorescent probes to measure the extent of fusion pore dilation. This identified that EC cells have a reduced fusion pore expansion that does not exceed 9nm in diameter. These results demonstrate that the small amounts of 5-HT released per fusion event in EC cells can be explained by a smaller fusion pore that limits 5-HT release capacity from individual vesicles.

LanguageEnglish
Pages1593-1600
Number of pages8
JournalJournal of Cellular Physiology
Volume231
Issue number7
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Raghupathi, Ravinarayan ; Jessup, Claire F. ; Lumsden, Amanda ; Keating, Damien J. / Fusion Pore Size Limits 5-HT Release From Single Enterochromaffin Cell Vesicles. In: Journal of Cellular Physiology. 2016 ; Vol. 231, No. 7. pp. 1593-1600.
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Fusion Pore Size Limits 5-HT Release From Single Enterochromaffin Cell Vesicles. / Raghupathi, Ravinarayan; Jessup, Claire F.; Lumsden, Amanda; Keating, Damien J.

In: Journal of Cellular Physiology, Vol. 231, No. 7, 01.07.2016, p. 1593-1600.

Research output: Contribution to journalArticle

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