Focal cerebral ischemia induces CRH mRNA in rat cerebral cortex and amygdala

Ma-Li Wong, Sara A. Loddick, Peter B. Bongiorno, Philip W. Gold, Nancy J. Rothwell, Julio Licinio

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CORTICOTROPIN-releasing hormone (CRH) antagonism has neuroprotective effects in models of ischemia. We examined CRH mRNA by in situ hybridization in a well-established rat model of focal cerebral ischemia caused by permanent middle cerebral artery occlusion (MCAo). In ischemic cortex CRH mRNA levels were elevated 2.6-fold 60min after MCAo, compared with sham operated animals. CRH mRNA was also induced in the amygdala, 60min following ischemia, in a pattern which was qualitatively different from that of sham operated animals. This rapid and profound increase in CRH mRNA levels during focal cerebral ischemia is likely to be associated with neurotoxicity, as CRH antagonism has been reported to cause a significant reduction in neuronal loss during ischemia.

Original languageEnglish
Pages (from-to)1785-1788
Number of pages4
Issue number13
Publication statusPublished - 1 Jan 1995


  • Amygdala
  • Cerebral cortex
  • Cerebral ischemia
  • Corticotropin-releasing hormone
  • Densitometry
  • In situ hybridization
  • Paraventricular hypothalamic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

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