Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes

Michael Hahne, Ute Jäger, Sandra Isenmann, Rupert Hallmann, Dietmar Vestweber

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Abstract

We have distinguished five TNF-α-inducible cell adhesion mechanisms on microvasculature-derived endothelioma cells of the mouse which mediate the binding of different types of leukocytes. Three of these mechanisms could be identified as the mouse homologs of ICAM-1, VCAM-1, and E-selectin, of which the latter was defined by the novel mAb 2 1KC10. The fourth TNF-α-inducible cell adhesion mechanism was blocked by antibodies specific for mouse P-selectin. We have recently shown that TNF-α stimulates the synthesis of P-selectin in mouse endothelioma cells (A. Weller, S. Isenmann, D. Vestweber. 1992. J. Biol. Chem. 267:15176-15183). Here we show that this stimulation leads to maximal cell surface expression levels within 4 h after stimulation while the same endothelioma cells are also able to upregulate P-selectin at the cell surface within minutes after stimulation with PMA. Both effects are additive. The fifth TNF-induced cell adhesion mechanism is defined by mediating the binding to the mouse monocyte/macrophage cell line J774. This adhesion mechanism is not inhibited by antibodies against any of the other four CAMs; it functions well at 7°C (in contrast to ICAM-1 and VCAM-1) and it is as active after 16 h of TNF induction as after 4 h (in contrast to E- and P-selectin). Furthermore, this new adhesion mechanism only functions on two of three endothelioma cell lines and is undetectable on the third, although ICAM-1, VCAM-1, E-selectin, and P-selectin could be demonstrated to function well on this cell line. Thus, in addition to the three known TNF-inducible CAMs, ICAM-1, VCAM-1, and E-selectin, also P-selectin and a fifth, as yet molecularly undefined cell adhesion mechanism, are TNF inducible at the cell surface of mouse endothelioma cells.

LanguageEnglish
Pages655-664
Number of pages10
JournalJournal of Cell Biology
Volume121
Issue number3
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hahne, Michael ; Jäger, Ute ; Isenmann, Sandra ; Hallmann, Rupert ; Vestweber, Dietmar. / Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes. In: Journal of Cell Biology. 1993 ; Vol. 121, No. 3. pp. 655-664.
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Five tumor necrosis factor-inducible cell adhesion mechanisms on the surface of mouse endothelioma cells mediate the binding of leukocytes. / Hahne, Michael; Jäger, Ute; Isenmann, Sandra; Hallmann, Rupert; Vestweber, Dietmar.

In: Journal of Cell Biology, Vol. 121, No. 3, 1993, p. 655-664.

Research output: Contribution to journalArticle

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