Fall in density, but not number of myenteric neurons and circular muscle nerve fibres in guinea-pig colon with ageing

C. J. Peck, S. D. Samsuria, Andrea Harrington, S. K. King, J. M. Hutson, B. R. Southwell

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27 Citations (Scopus)

Abstract

In guinea-pig ileum, ageing has been associated with a decrease in enteric neurons. This study examined guinea-pig colon and measured changes in gut dimensions, neuron size, density and ganglionic area. Changes in motor nerve fibres in the circular muscle were also measured. Myenteric neurons in whole-mount preparations of mid-colon from 2-week, 6-month, and 2-year-old guinea-pigs were labelled immunohistochemically with the neuronal marker human neuronal protein HuC/HuD, and numbers of neurons mm-2, neuronal size, ganglionic area mm-2, gut length, circumference and muscle thickness were measured. Corrected numbers of neurons mm-2 and ganglionic area mm-2 accounting for growth of the colon were calculated. Additionally, nerve fibres in circular muscle cross-sections were labelled with antibodies against nitric oxide synthase (NOS) and substance P (SP) and the density of nerve fibres in circular muscle was measured. The numbers of neurons mm-2 decreased by 56% (from 2 weeks to 2 years) with no change in neuron size. Total neuron numbers decreased by 19% (P = 0.14) when adjusted for changes in length and circumference with age. The percentage area of NOS- and SP-immunoreactive (IR) nerve fibres in the circular muscle decreased (P < 0.001), but the total area of NOS and SP-IR nerve fibres increased (P < 0.01) due to an age-related increase in muscle thickness. The density of myenteric neurons in guinea-pig mid-colon halved from 2 weeks to 2 years, but when the increase in colon dimensions was considered, the number of neurons decreased by only 19%. The percentage area of motor nerve fibres in the circular muscle decreased with no change in total volume of nerve fibres.

Original languageEnglish
JournalNeurogastroenterology and Motility
Volume21
Issue number10
DOIs
Publication statusPublished or Issued - 1 Oct 2009

Keywords

  • Ageing
  • Colon
  • Enteric neurons
  • Nitric oxide synthase
  • Substance P

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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