Failure to launch: Aberrant cumulus gene expression during oocyte in vitro maturation

Hannah M. Brown, Kylie R. Dunning, Melanie Sutton-McDowall, Robert B. Gilchrist, Jeremy G. Thompson, Darryl L. Russell

    Research output: Contribution to journalReview article

    17 Citations (Scopus)

    Abstract

    In vitro maturation (IVM) offers significant benefits for human infertility treatment and animal breeding, but this potential is yet to be fully realised due to reduced oocyte developmental competence in comparison with in vivo matured oocytes. Cumulus cells occupy an essential position in determining oocyte developmental competence. Here we have examined the areas of deficient gene expression, as determined within microarrays primarily from cumulus cells of mouse COCs, but also other species, between in vivo matured and in vitro matured oocytes. By retrospectively analysing the literature, directed by focussing on downregulated genes, we provide an insight as to why the in vitro cumulus cells fail to support full oocyte potential and dissect molecular pathways that have important roles in oocyte competence. We conclude that the roles of epidermal growth factor signalling, the expanded extracellular matrix, cumulus cell metabolism and the immune system are critical deficiencies in cumulus cells of IVM COCs.

    LanguageEnglish
    PagesR109-R120
    JournalReproduction
    Volume153
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 2017

    ASJC Scopus subject areas

    • Reproductive Medicine
    • Embryology
    • Endocrinology
    • Obstetrics and Gynaecology
    • Cell Biology

    Cite this

    Brown, H. M., Dunning, K. R., Sutton-McDowall, M., Gilchrist, R. B., Thompson, J. G., & Russell, D. L. (2017). Failure to launch: Aberrant cumulus gene expression during oocyte in vitro maturation. Reproduction, 153(3), R109-R120. https://doi.org/10.1530/REP-16-0426
    Brown, Hannah M. ; Dunning, Kylie R. ; Sutton-McDowall, Melanie ; Gilchrist, Robert B. ; Thompson, Jeremy G. ; Russell, Darryl L. / Failure to launch : Aberrant cumulus gene expression during oocyte in vitro maturation. In: Reproduction. 2017 ; Vol. 153, No. 3. pp. R109-R120.
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    Brown, HM, Dunning, KR, Sutton-McDowall, M, Gilchrist, RB, Thompson, JG & Russell, DL 2017, 'Failure to launch: Aberrant cumulus gene expression during oocyte in vitro maturation', Reproduction, vol. 153, no. 3, pp. R109-R120. https://doi.org/10.1530/REP-16-0426

    Failure to launch : Aberrant cumulus gene expression during oocyte in vitro maturation. / Brown, Hannah M.; Dunning, Kylie R.; Sutton-McDowall, Melanie; Gilchrist, Robert B.; Thompson, Jeremy G.; Russell, Darryl L.

    In: Reproduction, Vol. 153, No. 3, 01.01.2017, p. R109-R120.

    Research output: Contribution to journalReview article

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    AU - Dunning, Kylie R.

    AU - Sutton-McDowall, Melanie

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    Brown HM, Dunning KR, Sutton-McDowall M, Gilchrist RB, Thompson JG, Russell DL. Failure to launch: Aberrant cumulus gene expression during oocyte in vitro maturation. Reproduction. 2017 Jan 1;153(3):R109-R120. https://doi.org/10.1530/REP-16-0426