Expression of VEGF-A, otx homeobox and p53 family genes in proliferative vitreoretinopathy

Claudio Azzolini, Ilaria Pagani, Cristina Pirrone, Davide Borroni, Simone Donati, Muna Al Oum, Diana Pigni, Anna Maria Chiaravalli, Riccardo Vinciguerra, Francesca Simonelli, Giovanni Porta

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.

LanguageEnglish
Article number857380
JournalMediators of Inflammation
Volume2013
DOIs
Publication statusPublished - 1 Jan 2013

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Azzolini, Claudio ; Pagani, Ilaria ; Pirrone, Cristina ; Borroni, Davide ; Donati, Simone ; Al Oum, Muna ; Pigni, Diana ; Chiaravalli, Anna Maria ; Vinciguerra, Riccardo ; Simonelli, Francesca ; Porta, Giovanni. / Expression of VEGF-A, otx homeobox and p53 family genes in proliferative vitreoretinopathy. In: Mediators of Inflammation. 2013 ; Vol. 2013.
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abstract = "Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5{\%} to 10{\%} of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.",
author = "Claudio Azzolini and Ilaria Pagani and Cristina Pirrone and Davide Borroni and Simone Donati and {Al Oum}, Muna and Diana Pigni and Chiaravalli, {Anna Maria} and Riccardo Vinciguerra and Francesca Simonelli and Giovanni Porta",
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Azzolini, C, Pagani, I, Pirrone, C, Borroni, D, Donati, S, Al Oum, M, Pigni, D, Chiaravalli, AM, Vinciguerra, R, Simonelli, F & Porta, G 2013, 'Expression of VEGF-A, otx homeobox and p53 family genes in proliferative vitreoretinopathy', Mediators of Inflammation, vol. 2013, 857380. https://doi.org/10.1155/2013/857380

Expression of VEGF-A, otx homeobox and p53 family genes in proliferative vitreoretinopathy. / Azzolini, Claudio; Pagani, Ilaria; Pirrone, Cristina; Borroni, Davide; Donati, Simone; Al Oum, Muna; Pigni, Diana; Chiaravalli, Anna Maria; Vinciguerra, Riccardo; Simonelli, Francesca; Porta, Giovanni.

In: Mediators of Inflammation, Vol. 2013, 857380, 01.01.2013.

Research output: Contribution to journalArticle

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T1 - Expression of VEGF-A, otx homeobox and p53 family genes in proliferative vitreoretinopathy

AU - Azzolini, Claudio

AU - Pagani, Ilaria

AU - Pirrone, Cristina

AU - Borroni, Davide

AU - Donati, Simone

AU - Al Oum, Muna

AU - Pigni, Diana

AU - Chiaravalli, Anna Maria

AU - Vinciguerra, Riccardo

AU - Simonelli, Francesca

AU - Porta, Giovanni

PY - 2013/1/1

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N2 - Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.

AB - Introduction. Proliferative vitreoretinopathy (PVR) is a severe inflammatory complication of retinal detachment. Pathological epiretinal membranes grow on the retina surface leading to contraction, and surgery fails in 5% to 10% of the cases. We evaluated the expression of VEGF-A, Otx1, Otx2, Otx3, and p53 family members from PVR specimens to correlate their role in inducing or preventing the pathology. Methods. Twelve retinal samples were taken from patients affected by PVR during therapeutic retinectomies in vitreoretinal surgery. Gene expression was evaluated using quantitative real-time reverse transcriptase PCR analysis and immunohistochemistry, using four healthy human retinae as control. Result. Controls showed basal expression of all genes. PVR samples showed little or no expression of Otx1 and variable expression of VEGF-A, Otx2, Otx3, p53, and p63 genes. Significant correlation was found among VEGF-A, Otx2, p53, and p63 and between Otx1 and Otx3. Conclusions. Otx homeobox, p53 family, and VEGF-A genes are expressed in PVR human retina. We individuated two possible pathways (VEGF-A, Otx2, p53, p63 and Otx1 and Otx3) involved in PVR progression that could influence in different manners the course of the pathology. Individuating the genetic pathways of PVR represents a novel approach to PVR therapies.

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