Expression of corticotropin releasing hormone receptors type I and type II mRNA in suicide victims and controls

N. Hiroi, M. L. Wong, J. Licinio, C. Park, M. Young, P. W. Gold, G. P. Chrousos, S. R. Bornstein

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85 Citations (Scopus)

Abstract

Corticotropin-releasing hormone (CRH) is a key neuroendocrine factor implementing endocrine, immune and behavioral responses to stress. CRH exerts its action through two major receptors, CRH-R1 and CRH-R2. Recently novel non-peptidic antagonists directed against CRH-R1 or CRH-R2 have been proposed as promising agents in the treatment of depression, anxiety and eating disorder. However, so far the CRH-receptor system has not been widely' studied in humans. Therefore, we employed quantitative TaqMan PCR to analyze the expression and distribution of both CRH-R1 and CRH-R2 in human brain tissue and peripheral organs. Furthermore the expression of CRH receptors was analyzed for the first time in pituitaries of suicide victims by in situ hybridization and quantitative PCR. Our data demonstrated a different expression pattern in humans as compared to rodents. Both CRH-R1 and CRH-R2 were expressed in high amounts in the brain with the strongest expression in the pituitary. As described in rodents, however the CRH-R1 in human was the predominant receptor in the brain (82.7 ± 11.0%), whilst CRH-R2 was the predominant receptor in peripheral organs (77.0 ± 15.8%). There was a shift in the ratio of CRH-R1/R2 in the pituitaries of suicide victims. In conclusion, both CRH-R1 and CRH-R2 are widely expressed in human tissues with a distribution substantially different from rodents. Strong expression of both CRH-R1 and CRH-R2 in human pituitaries suggests that particularly under stress, activation of the HPA axis can be maintained through both receptors.

LanguageEnglish
Pages540-546
Number of pages7
JournalMolecular Psychiatry
Volume6
Issue number5
DOIs
Publication statusPublished - 1 Sep 2001

Keywords

  • CRH receptor
  • Human tissues
  • Pituitary
  • Suicide

ASJC Scopus subject areas

  • Molecular Biology
  • Psychiatry and Mental health
  • Cellular and Molecular Neuroscience

Cite this

Hiroi, N., Wong, M. L., Licinio, J., Park, C., Young, M., Gold, P. W., ... Bornstein, S. R. (2001). Expression of corticotropin releasing hormone receptors type I and type II mRNA in suicide victims and controls. Molecular Psychiatry, 6(5), 540-546. https://doi.org/10.1038/sj.mp.4000908