Evaluation of the use of attenuated total reflectance mid infrared spectroscopy to determine fatty acids in intact seeds of barley (Hordeum vulgare)

D. Cozzolino, S. Roumeliotis, J. Eglinton

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12 Citations (Scopus)


Attenuated total reflectance (ATR) mid-infrared (MIR) spectroscopy was evaluated to measure fatty acids (FA) and total lipids in intact seed barley (Hordeum vulgare) samples. Results from this study demonstrated the capability of ATR-MIR spectroscopy coupled with partial least squares (PLS) regression to measure FA non-destructively in intact barley seeds. The coefficients of determination in cross validation (R2) and the standard error of cross validation (SECV) were 0.76 and 0.004g100g-1 for stearic, 0.75 and 0.037g100g-1 for oleic, 0.45 and 0.02g100g-1 for palmitic acids, and 0.89 and 0.13g100g-1 for total lipids, respectively. The practical implications of this study showed that ATR-MIR spectroscopy confer valuable benefits for the grain industry and in particular in breeding programs as a non-destructive high throughput method.

Original languageEnglish
Pages (from-to)478-483
Number of pages6
JournalLWT - Food Science and Technology
Issue number2
Publication statusPublished - May 2014


  • Attenuated total reflectance
  • Barley
  • Fatty acids
  • Lipids
  • Mid infrared

ASJC Scopus subject areas

  • Food Science

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