Evaluation of the Stag3 gene and the synaptonemal complex in a rat model (as/as) for male infertility

Mònica Bayés, I. Prieto, J. Noguchi, J. L. Barbero, Luis Perez-Jurado

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14 Citations (Scopus)

Abstract

Affected males (as/as) from the mutant TT rat strain are sterile due to spermatogenesis impairment with meiotic arrest at the pachytene stage. The as locus is on rat chromosome 12, in a region that shows conserved synteny to cM 74-94 on mouse chromosome 5. Stag3, a new member of the stromalin protein family, is expressed specifically in testis and associates to the synaptonemal complex. Mouse Stag3 gene has been assigned to cM 78 on chromosome 5. In this study, we have characterized the rat Stag3 gene and examined it as a candidate for male infertility in as/as rats, The rat Stag3 cDNA is 4181 nucleotides long, contains a highly polymorphic hexanucleotide repeat in the coding region, and encodes a 1256 amino acid protein with 93 and 77% sequence identity to mouse and human Stag3 proteins, respectively. No mutations or differences in size or abundance of Stag3 mRNA were detected between as/as and control rats, suggesting that Stag3 is not responsible for the aspermic phenotype. In addition, immunohistochemistry with antibodies against SCP1 and SPC3 proteins suggest that the synaptonemal complex structures are not primarily affected in these rats.

Original languageEnglish
Pages (from-to)414-417
Number of pages4
JournalMolecular Reproduction and Development
Volume60
Issue number3
DOIs
Publication statusPublished or Issued - 22 Oct 2001
Externally publishedYes

Keywords

  • Candidate gene
  • Meiotic arrest
  • Mutation search
  • Sterility
  • Stromalin
  • Synteny

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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