Endosomal pH modulation by peptide-gold nanoparticle hybrids enables potent anti-inflammatory activity in phagocytic immune cells

Hong Yang, Lisa Kozicky, Aabida Saferali, Shan Yu Fung, Nicole Afacan, Bing Cai, Reza Falsafi, Erin Gill, Mingyao Liu, Tobias R. Kollmann, Robert Hancock, Laura M. Sly, Stuart E. Turvey

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Toll-like receptor (TLR) signaling plays a central role in the pathophysiology of many acute and chronic human inflammatory diseases, and pharmacological regulation of TLR responses is anticipated to be beneficial in many inflammatory conditions. Currently there are no specific TLR inhibitors in clinical use. To overcome this challenge, we have developed a nano-based TLR inhibitor (peptide-gold nanoparticle hybrids) that inhibits a broad spectrum of TLR responses. Through mechanistic studies, we established that specific peptide decorated-gold nanoparticles that display high cellular uptake in phagocytic immune cells modulate endosomal pH, leading to significant attenuation of signaling through multiple TLRs. Using a global transcriptomic approach, we defined the broad anti-inflammatory activity of the nanoparticle in human peripheral blood mononuclear cells. In vivo studies confirmed the beneficial immunomodulatory activity since treatment with the nanoparticle significantly reduced weight loss, improved the disease activity index, and ameliorated colonic inflammation in a murine model of intestinal inflammation. This work enhances our fundamental understanding of the role of peptide coatings on the nanoparticle surface in regulating innate immune signaling, and identifies specific peptide decorated nanoparticles that may represent a novel class of anti-inflammatory therapeutics for human inflammatory diseases.

LanguageEnglish
Pages90-102
Number of pages13
JournalBiomaterials
Volume111
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Anti-inflammatory therapeutics
  • Immune modulation
  • Inflammatory bowel disease
  • Nanoparticle
  • Peptide-conjugation
  • Toll-like receptor signaling

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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