Elevated IL-5 and IL-13 responses to egg proteins predate the introduction of egg in solid foods in infants with eczema

J. R. Metcalfe, N. D'Vaz, M. Makrides, M. S. Gold, P. Quinn, C. E. West, R. Loh, S. L. Prescott, D. J. Palmer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Egg allergy is a leading cause of food allergy in young infants; however, little is known about early allergen-specific T-cell responses which predate the presentation of egg allergy, and if these are altered by early egg exposure. Objective: To investigate the early T-cell responses to multiple egg proteins in relation to patterns of egg exposure and subsequent IgE-mediated egg allergy. Methods: Egg-specific T-cell cytokine responses (IL-5, IL-13, IL-10, IFNγ and TNFα) to ovomucoid (OM), ovalbumin (OVA), conalbumin (CON) and lysozyme (LYS) were measured in infants with eczema at 4months of age (n=40), before randomization to receive 'early egg' or a placebo as part of a randomized controlled trial (Australian New Zealand Clinical Trials Registry number 12609000415202) and at 12months of age (n=58), when IgE-mediated egg allergy was assessed by skin prick test and food challenge. Results: In 4-month-old infants, who had not directly ingested egg, those who subsequently developed egg allergy already had significantly higher Th2 cytokine responses to multiple egg allergens, particularly elevated IL-13 responses to OVA (P=0.004), OM (P=0.012) and LYS (P=0.003) and elevated IL-5 to the same antigens (P=0.031, 0.04 and 0.003, respectively). IL-13 responses (to OVA and LYS) and IL-5 responses (to LYS) at 4months significantly predicted egg allergy at 12months. All responses significantly declined with age in the egg-allergic infants, and this did not appear to be modified by 'early' introduction of egg. Conclusions & Clinical Relevance: Elevated egg-specific Th2 cytokine responses were established prior to egg ingestion at 4months and were not significantly altered by introduction of egg. Th2 responses at 4months of age predicted egg allergy at 12months, suggesting that this could be used as a biomarker to select infants for early prevention and management strategies.

LanguageEnglish
Pages308-316
Number of pages9
JournalClinical and Experimental Allergy
Volume46
Issue number2
DOIs
Publication statusPublished - 1 Feb 2016

Keywords

  • Allergy prevention
  • Cytokines
  • Eczema
  • Egg allergy
  • Egg protein
  • Infancy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Metcalfe, J. R. ; D'Vaz, N. ; Makrides, M. ; Gold, M. S. ; Quinn, P. ; West, C. E. ; Loh, R. ; Prescott, S. L. ; Palmer, D. J. / Elevated IL-5 and IL-13 responses to egg proteins predate the introduction of egg in solid foods in infants with eczema. In: Clinical and Experimental Allergy. 2016 ; Vol. 46, No. 2. pp. 308-316.
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abstract = "Background: Egg allergy is a leading cause of food allergy in young infants; however, little is known about early allergen-specific T-cell responses which predate the presentation of egg allergy, and if these are altered by early egg exposure. Objective: To investigate the early T-cell responses to multiple egg proteins in relation to patterns of egg exposure and subsequent IgE-mediated egg allergy. Methods: Egg-specific T-cell cytokine responses (IL-5, IL-13, IL-10, IFNγ and TNFα) to ovomucoid (OM), ovalbumin (OVA), conalbumin (CON) and lysozyme (LYS) were measured in infants with eczema at 4months of age (n=40), before randomization to receive 'early egg' or a placebo as part of a randomized controlled trial (Australian New Zealand Clinical Trials Registry number 12609000415202) and at 12months of age (n=58), when IgE-mediated egg allergy was assessed by skin prick test and food challenge. Results: In 4-month-old infants, who had not directly ingested egg, those who subsequently developed egg allergy already had significantly higher Th2 cytokine responses to multiple egg allergens, particularly elevated IL-13 responses to OVA (P=0.004), OM (P=0.012) and LYS (P=0.003) and elevated IL-5 to the same antigens (P=0.031, 0.04 and 0.003, respectively). IL-13 responses (to OVA and LYS) and IL-5 responses (to LYS) at 4months significantly predicted egg allergy at 12months. All responses significantly declined with age in the egg-allergic infants, and this did not appear to be modified by 'early' introduction of egg. Conclusions & Clinical Relevance: Elevated egg-specific Th2 cytokine responses were established prior to egg ingestion at 4months and were not significantly altered by introduction of egg. Th2 responses at 4months of age predicted egg allergy at 12months, suggesting that this could be used as a biomarker to select infants for early prevention and management strategies.",
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Elevated IL-5 and IL-13 responses to egg proteins predate the introduction of egg in solid foods in infants with eczema. / Metcalfe, J. R.; D'Vaz, N.; Makrides, M.; Gold, M. S.; Quinn, P.; West, C. E.; Loh, R.; Prescott, S. L.; Palmer, D. J.

In: Clinical and Experimental Allergy, Vol. 46, No. 2, 01.02.2016, p. 308-316.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated IL-5 and IL-13 responses to egg proteins predate the introduction of egg in solid foods in infants with eczema

AU - Metcalfe, J. R.

AU - D'Vaz, N.

AU - Makrides, M.

AU - Gold, M. S.

AU - Quinn, P.

AU - West, C. E.

AU - Loh, R.

AU - Prescott, S. L.

AU - Palmer, D. J.

PY - 2016/2/1

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N2 - Background: Egg allergy is a leading cause of food allergy in young infants; however, little is known about early allergen-specific T-cell responses which predate the presentation of egg allergy, and if these are altered by early egg exposure. Objective: To investigate the early T-cell responses to multiple egg proteins in relation to patterns of egg exposure and subsequent IgE-mediated egg allergy. Methods: Egg-specific T-cell cytokine responses (IL-5, IL-13, IL-10, IFNγ and TNFα) to ovomucoid (OM), ovalbumin (OVA), conalbumin (CON) and lysozyme (LYS) were measured in infants with eczema at 4months of age (n=40), before randomization to receive 'early egg' or a placebo as part of a randomized controlled trial (Australian New Zealand Clinical Trials Registry number 12609000415202) and at 12months of age (n=58), when IgE-mediated egg allergy was assessed by skin prick test and food challenge. Results: In 4-month-old infants, who had not directly ingested egg, those who subsequently developed egg allergy already had significantly higher Th2 cytokine responses to multiple egg allergens, particularly elevated IL-13 responses to OVA (P=0.004), OM (P=0.012) and LYS (P=0.003) and elevated IL-5 to the same antigens (P=0.031, 0.04 and 0.003, respectively). IL-13 responses (to OVA and LYS) and IL-5 responses (to LYS) at 4months significantly predicted egg allergy at 12months. All responses significantly declined with age in the egg-allergic infants, and this did not appear to be modified by 'early' introduction of egg. Conclusions & Clinical Relevance: Elevated egg-specific Th2 cytokine responses were established prior to egg ingestion at 4months and were not significantly altered by introduction of egg. Th2 responses at 4months of age predicted egg allergy at 12months, suggesting that this could be used as a biomarker to select infants for early prevention and management strategies.

AB - Background: Egg allergy is a leading cause of food allergy in young infants; however, little is known about early allergen-specific T-cell responses which predate the presentation of egg allergy, and if these are altered by early egg exposure. Objective: To investigate the early T-cell responses to multiple egg proteins in relation to patterns of egg exposure and subsequent IgE-mediated egg allergy. Methods: Egg-specific T-cell cytokine responses (IL-5, IL-13, IL-10, IFNγ and TNFα) to ovomucoid (OM), ovalbumin (OVA), conalbumin (CON) and lysozyme (LYS) were measured in infants with eczema at 4months of age (n=40), before randomization to receive 'early egg' or a placebo as part of a randomized controlled trial (Australian New Zealand Clinical Trials Registry number 12609000415202) and at 12months of age (n=58), when IgE-mediated egg allergy was assessed by skin prick test and food challenge. Results: In 4-month-old infants, who had not directly ingested egg, those who subsequently developed egg allergy already had significantly higher Th2 cytokine responses to multiple egg allergens, particularly elevated IL-13 responses to OVA (P=0.004), OM (P=0.012) and LYS (P=0.003) and elevated IL-5 to the same antigens (P=0.031, 0.04 and 0.003, respectively). IL-13 responses (to OVA and LYS) and IL-5 responses (to LYS) at 4months significantly predicted egg allergy at 12months. All responses significantly declined with age in the egg-allergic infants, and this did not appear to be modified by 'early' introduction of egg. Conclusions & Clinical Relevance: Elevated egg-specific Th2 cytokine responses were established prior to egg ingestion at 4months and were not significantly altered by introduction of egg. Th2 responses at 4months of age predicted egg allergy at 12months, suggesting that this could be used as a biomarker to select infants for early prevention and management strategies.

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KW - Cytokines

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KW - Egg protein

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